Summary
Current noise power spectra of the voltage-clamped (V=0)Necturus gallbladder, exposed to NaCl-Ringer's on both sides contained a relaxation noise component, which overlapped with a 1/f α noise component, with α being about 2. Substitution of all Na+ by K+ on either the serosal or mucosal side increased the relaxation as well as the 1/f α noise component considerably. InNecturus gallbladder both noise components are reduced by addition of 10mm 2,4,6-triaminopyrimidine (TAP) or 5mm of tetraethylammonium (TEA+) added to ification of the mucosal solution to pH 5 and lower. Fivemm of tetraethylammonium (TEA+) added to the mucosal solution, abolished K+ relaxation noise and decreased the 1/f α noise component. Applying a Cs+ concentration gradient across the epithelium did not yield relaxation noise. However, if Rb+ was substituted for all Na+ on one side, a Lorentzian noise component appeared in the spectrum. Its plateau was smaller than with KCl-Ringer's on the respective side. These data confirm the existence of fluctuating K+ channels in the apical membrane of theNecturus gallbladder. Furthermore it can be concluded that these channels have the permeability sequence K+>Rb+>Sc+. The inhibition of the fluctuations by mucosal acidification indicates the existence of acidic sites in the channel. The single-channel conductance was estimated to be between 6.5 and 40 pS.
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References
Armstrong, C.M. 1975. Potassium pores of nerve and muscle membranes.In: Membranes. A Series of Advances. Vol. 3. G. Eisenman, editor. Marcel Dekker, Inc., New York
Barry, P.H., Diamond, J.M. 1970. Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranes.J. Membrane Biol. 3:93
Chen, Y., Hill, T.L. 1973. Fluctuations and noise in kinetic systems. Application to K+ channels in the squid axon.Biophys. J. 13:1276
Claude, P. 1978. Morphological factors influencing transepithelial permeability: A model for the resistance of the zonula occludens.J. Membrane Biol. 39:219
Conti, F., DeFelice, L.J., Wanke, E. 1975. Potassium and sodium ion current noise in the membrane of the squid giant axon.J. Physiol. (London) 248:45
Curci, S., Frömter, E. 1979. Micropuncture of lateral intercellular spaces ofNecturus gallbladder to determine space fluid K+ concentration.Nature (London) 278:355
Dorset, D.L., Fishman, H.M. 1975. Excess electrical noise during current flow through porous membranes separating ionic solutions.J. Membrane Biol. 21:291
Drouin, H., The, R. 1969. The effect of reducing extracellular pH on the membrane currents of theRanvier node.Pfluegers Arch. 313:80
Fishman, H.M., Moore, L.E., Poussart, D.J.M. 1975. Potassiumion conduction noise in squid axon membrane.J. Membrane Biol. 24:305
Frömter, E. 1972. The route of passive ion movement through the epithelium ofNecturus gallbladder.J. Membrane Biol. 8:259
Frömter, E., Suzuki, K., Kottra, G., Kampmann, L. 1981. The paracellular shunt conductance ofNecturus gallbladder epithelium. Comparison of measurements obtained by cable analysis with measurements obtained by a new approach based on intracellular impedance analysis.In: Epithelial Ion and Water Transport. A.D.C. Macknight, editor. Raven Press, New York
Gögelein, H., Van Driessche, W. 1981. The effect of electrical gradients on current fluctuations and impedance recorded fromNecturus gallbladder.J. Membrane Biol. 60:99
Gordon, L.G.M. 1978. Cellular and shunt conductance of toad bladder epithelium.J. Membrane Biol. 44:309
Green, M.E. 1976. Diffusion and 1/f noise.J. Membrane Biol. 28:181
Hagiwara, S., Eaton, D.C., Stuart, A.E., Rosenthal, N.P. 1972. Cation selectivity of the resting membrane of squid axon.J. Membrane Biol. 9:373
Hénin, S., Cremaschi, D. 1975. Transcellular ion route in rabbit gallbladder. Electric properties of the epithelial cells.Pfluegers Arch. 355:125
Hille, B. 1973. Potassium channels in myelinated nerve. Selective permeability to small cations.J. Gen. Physiol. 61:669
Lindemann, B., Van Driessche, W. Sodium-specific membrane channels of frog skin are pores: Current fluctuations reveal high turnover.Science 195:292
Moore, L.E., Fishman, H.M., Poussart, D.J.M. 1979. Chemically induced K+ conduction noise in squid axon.J. Membrane Biol. 47:99
Moreno, J.H. 1975. Blockage of gallbladder tight junction cation-selective channels by 2,4,6-triaminopyrimidinium (TAP).J. Gen. Physiol. 66:97
Reuss, L. 1979. Electrical properties of the cellular transepithelial pathway inNecturus gallbladder: III. Ionic permeability of the basolateral cell membrane.J. Membrane Biol. 47:239
Reuss, L., Finn, A.L. 1975a. Electrical properties of the cellular transepithelial pathway inNecturus gallbladder. I. Circuit analysis and steady-state effects of mucosal solution ionic substitutions.J. Membrane Biol. 25:115
Reuss, L., Finn, A.L. 1975b. Electrical properties of the cellular transepithelial pathway inNecturus gallbladder. II. Ionic permeability of the apical cell membrane.J. Membrane Biol. 25:141
Reuss, L., Grady, T.P. 1979. Triaminopyrimidinium (TAP+) blocks luminal membrane K conductance inNecturus gallbladder epithelium.J. Membrane Biol. 48:285
Reuss, L., Weinman, S.A. 1979. Intracellular ionic activities and transmembrane electrochemical potential differences in gallbladder epithelium.J. Membrane Biol. 49:345
Spring, K.R., Hope, A. 1979. Dimensions of cells and lateral intercellular spaces in livingNecturus gallbladder.Fed. Proc. 38:128
Stevens, C.F. 1972. Inferences about membrane properties from electrical noise measurements.Biophys. J. 12:1028
Van den Berg, R.J., Siebenga, E., De Bruin, G. 1977. Potassium ion noise currents and inactivation in voltage-clamped node ofRanvier.Nature (London) 265:177
Van Driessche, W., Gögelein, H. 1978. Potassium channels in the apical membrane of the toad gallbladder.Nature (London) 275:665
Van Driessche, W., Gögelein, H. 1980. Attenuation of current and voltage noise signals recorded from epithelia.J. Theor. Biol. 86:629
Van Driessche, W., Lindemann, B. 1978. Low-noise amplification of voltage and current fluctuations arising in epithelia.Rev. Sci. Instrum. 49:52
Van Driessche, W., Zeiske, W. 1980a. Spontaneous fluctuations of potassium channels in the apical membrane of frog skin.J. Physiol. (London) 299:101
Van Driessche, W., Zeiske, W. 1980b. Ba2+-induced conductance fluctuations of spontaneously fluctuating K+ channels in the apical membrane of frog skin (Rana temporaria).J. Membrane Biol. 56:31
Van Os, C.H., Slegers, J.F.G. 1975. The electrical potential profile of gallbladder epithelium.J. Membrane Biol. 24:341
Verveen, A.A., De Felice, L.J. 1974. Membrane noise.Prog. Biophys. Mol. Biol. 28:189
Wanke, E., DeFelice, L.J., Conti, F. 1974. Voltage noise, current noise and impedance in space clamped squid giant axon.Pfluegers Arch. 347:63
Wright, E.M., Diamond, J.M. 1968. Effects of pH and polyvalent cations on the selective permeability of gall-bladder epithelium to monovalent ions.Biochim. Biophys. Acta. 163:57
Zeuthen, T. 1978. Intracellular gradients of ion activities in the epithelial cells of theNecturus gallbladder recorded with ion-selective microelectrodes.J. Membrane Biol. 39:185
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Gögelein, H., Van Driessche, W. Noise analysis of the K+ current through the apical membrane ofNecturus gallbladder. J. Membrain Biol. 60, 187–198 (1981). https://doi.org/10.1007/BF01992557
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DOI: https://doi.org/10.1007/BF01992557