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Experimental mast cell activation improves connective tissue repair in the perforated rat mesentery

  • Inflammation and Immunomodulation
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Abstract

The participation of mast cells in connective tissue repair was studied using the perforated-rat-mesentery model. Perforation of mesenteric membranes was performed during laparotomy of anesthetized Sprague-Dawley rats. Laparotomy significantly reduced the histamine content of the mesenteric membranes on day 1 postoperatively and perforation as such reduced the histamine content even more on days 1–10. Mast cell activation induced by a single intraperitoneal injection of Compound 48/80 two days prior to operation, significantly improved healing on days 5–7 postoperatively. Daily injections of Compound 48/80 for 5 days prior to operation showed significantly better healing compared to such injections for five days postoperatively. Administration of lupitidine, a long acting histamine H2-receptor antagonist, two times daily starting on the day of 48/80 injection to day 4 after operation did not apparently affect healing.

The results indicate that mast cells may be activated during normal wound healing and that a preoperative, pharmacological activation improves healing. Furthermore, histamine does not seem to be of major importance for the beneficial effect of such mast cell activation on connective tissue repair.

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Authors and Affiliations

  1. Department of Pathology II, University Hospital, S-581 85, Linköping, Sweden

    L. Franzén, R. Ghassemifar & P. Malcherek

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  1. L. Franzén
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  2. R. Ghassemifar
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  3. P. Malcherek
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Franzén, L., Ghassemifar, R. & Malcherek, P. Experimental mast cell activation improves connective tissue repair in the perforated rat mesentery. Agents and Actions 33, 371–377 (1991). https://doi.org/10.1007/BF01986588

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