Summary
A mast-cell activator, compound 48/80, causes proliferation of mesenchymal cells in the mesentery of rats. Its effect on W/W vmice deficient in mast cells was tested to determine whether the proliferation is mediated in the degranulation of mast cells. Incorporation of [3H]thymidine into mesenchymal cells in the mesentery of these mice with or without compound 48/80 was very small compared to their normal litter mates. However, bone marrow transplantation markedly enhanced the effect of compound 48/80, and resulted in an incorporation of [3H]thymidine almost comparable to that observed in normal mice. Our results provide evidence that mesenchymal cell proliferation is caused by a product secreted by mast cells when stimulated by compound 48/80.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. Garland Publishing, Inc., New York
Crowle PK (1983) Mucosal mast cell reconstitution and Nippostrongylus brasiliensis rejection by W/W vmice. J Parasitol 69:66–69
Franzén L, Norrby K (1977) Different proliferative responsiveness of fibroblasts and mesothelial cells in the rat mesentery following administration of Compound 48/80 at various hours of the day. Virchows Arch B [Cell Pathol] 24:91–99
Franzén L, Norrby K (1980) Local mitogenic effect of tissue mast cell section. Cell Tissue Kinet 13:635–642
Fujimoto S, Yamamoto K, Arashidani K, Hayabuchi I, Yoshizuka M, Nomiyama T (1982) Endothelial specific granules in the umbilical veins of the postnatal rabbit. Cell Tissue Res 227:509–518
Ha TY, Reed ND, Crowle PK (1983) Delayed expulsion of adult Trichinella spiralis by mast-cell-deficient W/W vmice. Infect Immun 41:445–447
Hatanaka K, Kitamura Y, Nishimune Y (1979) Local development of mast cells from bone marrow-derived precursors in the skin of mice. Blood 53:142–147
Hatanaka K, Minamiyama M, Tanaka K, Taguchi T, Tajima S, Kitamura Y, Yamamoto A (1985) High susceptibility to ADP-induced thrombus formation in mast cell-deficient W/W vmice. Thromb Res 40:453–464
Kitamura Y, Go S, Hatanaka K (1978) Decrease of mast cells in W/W vmice and their increase by bone marrow transplantation. Blood 52:447–452
Mitchell LA, Wescott RB, Perryman LE (1983) Kinetics of expulsion of the nematode, Nippostrongylus brasiliensis, in mast-cell-deficient W/W vmice. Parasite Immunol 5:1–12
Morrison DC, Roser JF, Henson PM, Cochrane CG (1974) Activation of rat mast cells by low molecular weight stimuli. J Immunol 112:573–582
Norrby K (1985) Evidence of mast-cell histamine being mitogenic in intact tissue. Agent Action 16:287–290
Norrby K, Enerbäck L, Franzén L (1976) Mast cell activation and tissue cell proliferation. Cell Tissue Res 170:289–303
Poon KC, Liu PI, Spicer SS (1981) Mast cell degranulation in beige mice with the Chediak-Higashi defect. Am J Pathol 104:142–149
Roche WR (1985) Mast cells and tumors: The specific enhancement of tumor proliferation in vitro. Am J Pathol 119:57–64
Author information
Authors and Affiliations
Additional information
Supported by a Grant-in-Aid for Scientific Research, No. 366, from the Japanese Ministry of Health and Welfare
The authors are indebted to Drs. Motomu Minamiyama and Yukio Hirata for valuable advices, and to Miss Mitsuko Inoue for technical assistance
Rights and permissions
About this article
Cite this article
Hatanaka, K., Imakita, M., Go, S. et al. Effects of degranulation of mast cells on proliferation of mesenchymal cells in the mesentery of mice. Cell Tissue Res. 246, 53–56 (1986). https://doi.org/10.1007/BF00218998
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218998