Summary
Lipid production of the oleaginous yeastApiotrichum curvatum was studied in wheypermeate to determine optimum operation conditions in this medium. Studies on the influence of the carbon to nitrogen ratio (C/N-ratio) of the growth medium on lipid production in continuous cultures demonstrated that cellular lipid content in wheypermeate remained constant at 22% of the cell dry weight up to a C/N-ratio of about 25. The maximal dilution rate at which all lactose is consumed in wheypermeate with excess nitrogen was found to be 0.073 h-1. At C/N-ratios higher than 25–30 lipid content gradually increased to nearly 50% at C/N=70 and the maximal obtainable dilution rate decreased to 0.02 h-1 at C/N=70. From these studies it could be derived that maximal lipid production rates can be obtained at C/N-ratios of 30–35 in wheypermeate. Since the C/N-ratio of wheypermeate normally has a value between 70 and 101, some additional nitrogen is required to optimize the lipid production rate. Lipid production rates ofA. curvatum in wheypermeate were compared in four different culture modes: batch, fed-batch, continuous and partial recycling cultures. Highest lipid production rates were achieved in culture modes with high cell densities. A lipid production rate of nearly 1 g/l/h was reached in a partial recycling culture. It was calculated that by using this cultivation technique lipid production rates of even 2.9 g/l/h may be reached when the supply of oxygen can be optimized.
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Abbreviations
- C/N-ratio:
-
carbon to nitrogen ratio of the growth medium (g/g)
- C/Ncrit :
-
C/N-ratio at which there is just enough nitrogen to allow all carbon source to be converted to biomass
- D:
-
dilution rate=volume of incoming medium per unit time/volume of medium in the culture vessel (h-1)
- Dmax :
-
maximum dilution rate (h-1)
- DW:
-
cell dry weight
- L:
-
lipid yield (g storage lipid/g carbon source)
- μ:
-
specific growth rate (h-1)
- μmax :
-
maximum specific growth rate (h-1)
- QL :
-
lipid production rate (g/l/h)
- Yi :
-
molecular fraction of carbon substrate that is converted to storage carbohydrate (C-mol/C-mol)
- Yls :
-
maximal amount of storage lipid that can be produced per mol carbon source (C-mol/C-mol)
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Ykema, A., Verbree, E.C., Kater, M.M. et al. Optimization of lipid production in the oleaginous yeastApiotrichum curvatum in wheypermeate. Appl Microbiol Biotechnol 29, 211–218 (1988). https://doi.org/10.1007/BF01982904
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DOI: https://doi.org/10.1007/BF01982904