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A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte

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Abstract

Using isolated rat hepatocytes the biochemical effects of hydrazine have been investigated using both conventional assay techniques and high resolution proton NMR. High resolution proton NMR revealed that hydrazine caused a significant increase in alanine and lactate levels in the incubation buffer, whereas levels of β-hydroxybutyrate were decreased. NMR also detected metabolites of hydrazine notably acetylhydrazine and a cyclised hydrazone formed with α-ketoglutarate. Changes were detected in NADH and NADPH, ATP, succinate dehydrogenase (SDH) and total non-protein sulphydryl groups (TNPSH). However, the changes in pyridine nucleotides occurred at higher concentrations than those affecting succinate dehydrogenase and ATP. Similarly, the depletion of TNPSH occurred at a higher concentration and with a different time course to that seen with ATP depletion and inhibition of succinate dehydrogenase.

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Authors and Affiliations

  1. Department of Toxicology, London School of Pharmacy, 29/39 Brunswick Square, WC1N 1AX, London, UK

    S. Ghatineh, W. Morgan & J. A. Timbrell

  2. Department of Biophysics, The Hunterian Institute, The Royal College of Surgeons, 35/45 Lincoln's Inn Fields, London, UK

    N. E. Preece

Authors
  1. S. Ghatineh
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  2. W. Morgan
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  3. N. E. Preece
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  4. J. A. Timbrell
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Ghatineh, S., Morgan, W., Preece, N.E. et al. A biochemical and NMR spectroscopic study of hydrazine in the isolated rat hepatocyte. Arch Toxicol 66, 660–668 (1992). https://doi.org/10.1007/BF01981506

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  • DOI: https://doi.org/10.1007/BF01981506

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