Abstract
Treatment for 11–13 weeks with a cholesterol-rich diet induced increases in free and esterified serum cholesterol. There was also an increase in free cholesterol of peritoneal macrophages. A 2.2 times rise in the cholesterol to phospholipid ratio of plasma membranes occurred in cholesterol-enriched macrophages. No changes were observed in phagolysosomes. Cholesterol-enriched macrophages showed a 35.8% inhibition of latex particles phagocytosis. When lipid droplets were substituted for latex the inhibition was 81.7%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
D. Chapman,Some Recent Studies of Lipids, Lipidcholesterol and Membrane Systems, in: D. Chapman and D.H.F. Wallach,Biological Membranes, vol. 2 (Academic Press, New York 1973), p. 91–144.
J. De Gier, J.G. Marderslot and L.L.M. Van Deenen,Lipid Composition and Permeability of Liposomes, Biochem. biophys. Acta150, 666–675 (1968).
R.A. Klein, M.J. Moore and M.W. Smith,Selective Diffusion of Neutral Amino Acids Across Lipid Bilayers, Biochem. biophys. Acta233, 420–433 (1971).
J.M. Boggs and J.C. Hsia,Effect of Cholesterol and Water on the Rigidity and Order of Phosphatidylcholine Bilayers, Biochim. biophys. Acta290, 32–42 (1972).
J. Kroes and R. Ostwald,Eruthrocyte Membranes-effect of Increased Cholesterol Content on Permeability, Biochim. biophys. Acta249, 647–650 (1971).
M. Poznansky, D. Kikwood and A.K. Solomon,Modulation of Red Cell K + Transport by Membrane Lipids, Biochem. biophys. Acta330, 351–355 (1973).
F. Feo, R.A. Canuto, R. Garcea and L. Gabriel,Effect of Cholesterol Content on Some Physical and Functional Properties of Mitochondria Isolated from Adult Rat Liver, Fetal Liver, Cholesterol-enriched Liver and Hepatomas AH-130, 3924A, and 5123, Biochim. biophys. Acta (in press).
M.U. Dianzani, F. Feo, G.A. Rita and M.F. Zuretti,The Role of Cholesterol in Biological Membranes, in: G.P. Velo, D.A. Willoughby and J.P. Giroud,Future Trends in Inflammation, (Piccin Medical Books, Brescia 1974), p. 385–404.
Z.A. Cohn,Some Aspects of Macrophage Membranes, in: I.H. Lepow and P.A. Ward,Inflammation. Mechanisms and Control, (Academic Press, New York 1972), p. 71–81.
S. Gordon and Z.A. Cohn,The Macrophage, Int. Rev. Cytol.36, 171–214 (1973).
Z. Werb and Z.A. Cohn,Plasma Membrane Synthesis in the Macrophage Following Phagocytosis of Polystyrene Latex Particles, J. Biol. Chem.247, 2439–2446 (1972).
Z. Werb and Z.A. Cohn,Cholesterol Metabolism in the Macrophage. II. Alteration of Subcellular Exchangeable Cholesterol Compartments and Exchange in Other Cell Types, J. exp. med.134, 1570–1590 (1971).
N.R. Di Luzio and S.J. Riggi,The Development of a Lipid Emulsion for the Measurement of Reticuloendothelial Function, J. Reticuloendothel. Soc.1, 136–145 (1964).
Y.H. Chang,Studies on Phagocytosis. I. Uptake of Radio-iodinated ( 131 I) Human Serum Albumin as a Measure of the Degree of Phagocytosis in vitro, Exp. Cell Res.54, 42–48 (1969).
L. Warren, M.C. Glick and M.K. Nass,Membranes of Animal Cells. I. Methods of Isolation of the Surface Membrane. J. Cell Physiol.68, 269–287 (1966).
C.D. De Duve, B.C. Pressman, R. Gianetto, R. Wattiaux and F. Appelmans,Tissue Fractionation Studies. 6. Intracellular Distribution Patterns of Enzymes in the Rat Liver Tissue, Biochem. JitJ.60, 604–617 (1955).
A.H. Phillips and R.G. Langdon,Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization and Kinetic Studies, J. biol. Chem.189, 379–386 (1951).
L.A. Heppel and R.J. Hilmoe,5′-Nucleotidases, in: S.P. Colowick and N.O. Kaplan,Methods in Enzymology, vol. 2 (Academic Press, New York 1955), p. 546–550.
Z. Sellinger, H. Beaufay, P. Jacques, A. Doyen and C.D. De Duve,Tissue Fractionation Studies. 15. Intracellular Distribution and Properties of β-N-Acetylglucosaminidase and β-Galactosidase in Rat Liver, Biochem. J.74, 450–456 (1960).
G.W. Löhr and H.D. Waller,Glucose-6-phosphate Dehydrogenase, in: H.V. Bergmeyer,Methods of Enzymatic Analysis, vol. 2 (Academic Press, New York 1974), p. 636–643.
F. Feo, R.A. Canuto, G. Bertone, R. Garcea and P. Pani,Cholesterol and Phospholipid Composition of Mitochondria and Microsomes Isolated from Morris Hepatoma 5123 and Rat Liver, FEBS Letters 33, 229–232 (1973).
I. Gutmann and A.W. Wahlefeld,L-(+)-Lactate Determination with Lactate Dehydrogenase and NAD, in: H.V. Bergmeyer,Methods of Enzymatic Analysis, vol. 3, (Academic Press, New York 1974), p. 1464–1468.
G.Y.N. Iyer, M.F. Islam and J.H. Quastel,Biochemical Aspects of Phagocytosis, Nature192, 535–541 (1961).
A.J. Sbarra and M.L. Karnovky,The Biochemical Bases of Phagocytosis. I. Metabolic Changes During Ingestion of Particles by Polimorphonuclear Leukocytes, J. biol. Chem.234, 1355–1362 (1959).
F. Rossi and M. Zatti,Changes in Metabolic Pattern of Polymorphonuclear Leucocytes During Phagocytosis, Br. J. exp. Pathol.45, 548–559 (1964).
R.R. Berger and M.L. Karnovsky,Biochemical Basis of Phagocytosis. V. Effect of Phagocytosis on Cellular Uptake of Extracellular Fluid and on the Intracellular Pool of L-alfa-Glycerophosphate, Fed. Proc.25, 840–845 (1966).
I. Carr,The Macrophage. A Review of Ultrastructure and Function (Academic Press, New York 1973), p. 61–72.
S. Rotten, J. Yashouv, Z. Ne'eman and S. Razin,Cholesterol in Mycoplasma Membranes. Composition, Ultrastructure and Biological Properties of Membranes from Mycoplasma Mycoides var. Capri Cells Adapted to Grow with Low Cholesterol Concentration, Biochim. biophys. Acta323, 495–508 (1973).
C. Mailer, C.P.S. Taylor, S. Schreier-Muccillo and I.C.P. Smith,The Influence of Cholesterol on Molecular Motion in Egg Lecithin Bilayers — A Variable-Frequency Electron Spin Resonance Study of a Cholestane Probe, Arch. Biochem. Biophys.163, 671–678 (1974).
J.M. Graham and C. Green,The Properties of Mitochondria Enriched in vitro with Cholesterol, Eur. J. Biochem.12, 58–66 (1970).
A.C. Worthing and J. Jeffner,Treatment of Experimental Data (Wiley, New York 1943).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Feo, F., Canuto, R.A., Torrielli, M.V. et al. Effect of a cholesterol-rich diet on cholesterol content and phagocytic activity of rat macrophages. Agents and Actions 6, 135–142 (1976). https://doi.org/10.1007/BF01972197
Issue Date:
DOI: https://doi.org/10.1007/BF01972197