Summary
The distribution of gamma-aminobutyric acid (GABA) in the rat kidney was examined by immunocytochemical techniques. GABA-like immunoreactivity (GABA-LI) was predominantly confined to the renal tubules, including the ascending parts of the distal tubules, and the loops of Henle, the collecting tubules and ducts, and the connective parts of the convoluted tubules. In GABA-positive cortical tubules, about half of the epithelial cells were labelled. The labelled cell type showed the ultrastructural features of principal cells. Depolarizing stimulation by ouabain and high K+ concentration evoked the efflux of endogenous GABA from kidney slices. The present findings, along with previous results, suggest that GABA released from renal tubular epithelium, and transported with the urine, might be involved in the modulation of contractility in the urinary tract.
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References
Erdö, S. L., Trends pharmac. Sci.6 (1985) 205.
Tanaka, C., Life Sci.30 (1985) 2221.
Erdö, S. L., and Bowery, N. G. (Eds), GABAergic Mechanisms in the Mammalian Periphery. Raven Press, New York 1989.
Erdö, S. L. and Kiss, B., in: GABAergic Mechanisms in the Mammalian Periphery, pp. 9–17. Eds S. L. Erdö and N. G. Bowery. Raven Press, New York 1986.
Erdö, S. L., and Wolff, J. R., J. Neurochem.34 (1990) 363.
Zachmann, M. P., Tocci, P., and Nyhan, W. L., J. biol. Chem.241 (1966) 1355.
Whelan, D. T., Scriver C. R., and Mohyuddin, F., Nature224 (1969) 916.
Haber, B., Kuriyama, K. and Roberts, E., Biochem. Pharmac.19 (1970) 1119.
Lancaster, G., Mohyuddin, F., Scriver, C. R., and Whelan, D. T., Biochim. biophys. Acta297 (1973) 229.
Wu, J.-Y., Chude, O., Wein, J., and Roberts, E., J. Neurochem.30 (1978) 849.
Goodyer, P. R., Lancaster, G., Villeneuve, M., and Scriver, C. R., Biochim. biophys. Acta633 (1980) 191.
Goodyer P. R., Mills, M., and Scriver, C. R., Biochim. biophys. Acta716 (1982) 348.
Somogyi, P., and Hodgson, A. J., J. Histochem. Cytochem.33 (1985) 249.
Hodgson, A. J., Penke, B., Erdei, A., Chubb, I. W. E., and Somogyi, P., J. Histochem. Cytochem.33 (1985) 229.
Hepler, J. R., Petrusz, P., and Rustioni, A., J. Histochem. Cytochem.34 (1986) 110.
Sternberger, J. A., Immunocytochemistry, 2nd edn., Wiley and Sons, New York 1979.
Erdö, S. L., and Wolff, J. R., Neurosci. Res. Commun.4 (1989) 51.
Ernst, S. A., J. Cell. Biol.66 (1975) 586.
Siegel, G. J., Holm, C., Schreiber, J. H., Desmond, T., and Ernst, S. A., J. Histochem. Cytochem.32 (1984) 1309.
Tallan, H. H., Moore, S., and Stein W. H., J. biol. Chem.,221 (1954) 927.
Goodyer, P. R., Rozen, R. & Scriver, C. R. Biochim. biophys. Acta818 (1985) 45.
Sidhu, H. S., and Wood, J. D., Experientia45 (1989) 726.
Erdö, S. L., Mione, M. C., Amenta, F., and Wolff, J. R., Br. J. Pharmac.96 (1989) 313.
Taniyama, K., Kusunoki, M., and Tanaka, C., Eur. J. Pharmac.89 (1983) 163.
Maggi, C. A., Santicioli, P. and Meli, A., J. Autonom. Pharmac.5 (1985) 55.
Maggi, C. A., Santicioli, P., and Meli, A., J. Autonom. Pharmac.5 (1985) 131.
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Erdö, S.L., Dobó, E., Párducz, A. et al. Releasable GABA in tubular epithelium of rat kidney. Experientia 47, 227–229 (1991). https://doi.org/10.1007/BF01958142
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DOI: https://doi.org/10.1007/BF01958142