Summary
The present study demonstrates that a) serum based culture medium degrades125I insulin; b) heat in-activation of serum (1 h, 56°C) inhibits its proteolytic activity leading to the recovery of more insulin secreted by islets cultured in the presence of high glucose concentration alone or with glucagon; c) aprotinin also favoured the accumulation of secreted insulin by protecting the hormone from a residual degradative capacity of the heat treated serum.
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References
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This work was supported by a grant (No. 71 5 426-2) from the INSERM and by the CNRS.
These results have been presented at the V International Congress of Endocrinology. Hamburg, July 18–24, 1976.
Acknowledgments. We should like to thank Mrs E. Gammelgard and K. Christensen for their technical assistance.
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Kedinger, M., Moody, A., Launay, J.F. et al. Establishment of serum based medium essentially free of proteolytic activity for the culture of mouse pancreatic islets. Experientia 33, 972–973 (1977). https://doi.org/10.1007/BF01951312
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DOI: https://doi.org/10.1007/BF01951312