Abstract
The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
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References
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Data Transformation and calculation of UC50 and IC50 data (DOC 3676 kb)
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Nicholls, D.G. (2018). Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells. In: Palmeira, C., Moreno, A. (eds) Mitochondrial Bioenergetics. Methods in Molecular Biology, vol 1782. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7831-1_7
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DOI: https://doi.org/10.1007/978-1-4939-7831-1_7
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