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Retention of noradrenaline-3H in brain and preferential extraction of labeled metabolites by glutaraldehyde fixation

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Résumé

Trois heures après une injection i.v. de noradrénaline tritée (NA−3H) chez le rat nouveau-né, moins de 5% de la radioactivité mesurée dans le cerveau correspond à cette amine marquée. Par contre, lorsque la monoamine oxydase et la catéchol-O-méthyl transférase sont inhibées, la NA−3H constitue 73% de la radioactivité cérébrale. Dans ces conditions, l'extraction de la radioactivité par la fixation au glutaraldéhyde 3.5% dans le tampon phosphate diffère également de façon significative et indique qu'une fraction majeure de la NA−3H est liée au tissu par le glutaraldéhyde, tandis que les métabolites marqués sont préférentiellement extraits.

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Authors and Affiliations

  1. Centre de recherche en sciences neurologiques, Université de Montréal, BP 6208, H3C 3T8, Montréal, Québec, Canada

    L. Descarries & J. C. Dupin

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  1. L. Descarries
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  2. J. C. Dupin
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Descarries, L., Dupin, J.C. Retention of noradrenaline-3H in brain and preferential extraction of labeled metabolites by glutaraldehyde fixation. Experientia 30, 1164–1165 (1974). https://doi.org/10.1007/BF01923667

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