Abstract
clinically healthy SCID mice were infected intratracheally withPneumocystis carinii (PC) of human origin. The data obtained provides unambiguous evidence that progressive multiplication of PC organisms of human origin takes place in the lungs of experimentally infected animals. SCID mice that were infected with human-derived PC also revealed a markedly greater number of mouse PC organisms in their lungs than the controls. All the SCID recipients of human PC died by day 65 post infection, whereas the controls, housed under identical conditions, started dying significantly later due to severe mouse pneumocystosis. This animal model could be used for the maintenance and propagation of human PC, and for evaluating strategies for treating human pneumocystosis.
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References
Mills, T., Rev. infect. Dis.8 (1986) 1001.
Salfelder, K., and Schwarz, J., Am. J. Dis. Child.114 (1967) 693.
Murray, J. F. et al., New Engl. J. Med.310 (1984) 1682.
Cushion, M. T., and Walzer, P. D., Infect. Immun.44 (1984) 245.
Bartlett, M. S., Verbanc, P. A., and Smith, J. W., J. clin. Microbiol.10 (1979) 796.
Pifer, L., Hughes, W. T., and Murphy, M. J., Ped. Res.II (1977) 305.
Frenkel, J. K., Good, J. T., and Schultz, J. A., Lab. Invest.15 (1966) 1559.
Walzer, P. D., in:Pneumocystis carinii infections. Ed. L. S. Young. Marcel Dekker Inc., New York 1984.
Sheldon, W. H., J. exp. Med.110 (1959) 147.
Sethi, K. K., Salfelder, K., and Schwarz, J., Mycopath. Mycol. appl. (1968) 121.
Walzer, P., Schnelle, V., Armstrong, D., and Rosen, P. P., Science,197 (1977) 177.
Bosma, G. C., Custer, R. P., and Bosma, M. J., Nature301 (1983) 527.
Walzer, P. D., et al., Infect. Immun.57 (1989) 62.
Sundberg, J. P., Burnstein, T., Shultz, L. D., and Bedigiam, H., Lab. Animal Sci.39 (1989) 213.
Bartlett, M. S., et al., J. clin. Microbiol.26 (1988) 1100.
Kovacs, J. A. et al., J. Immun.140 (1988) 2023.
Sethi, K. K., in: Progress in AIDS Research in the Federal Republic of Germany, pp. 283–289. MMV Medizin Verlag, München 1990.
Sethi, K. K., Ärztl. Lab.36 (1990) 116.
Goldman, M., in: Fluorescence Antibody Methods. Ed. M. Goldman. Academic Press, New York.
Grocott, R. G., Am. J. clin. Path.25 (1955) 175.
Cordell, J. L., et al., J. Histochem. Cytochem.32 (1984) 219.
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Sethi, K.K. Multiplication of human-derivedPneumocystis carinii in severe combined immunodeficient (SCID) mice. Experientia 48, 63–67 (1992). https://doi.org/10.1007/BF01923610
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DOI: https://doi.org/10.1007/BF01923610