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Effects of POCA on metabolism and function in the ischemic rat heart

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Summary

Sodium 2-[5-(4-chlorophenyl)-pentyl]-oxirane-2-carboxylate (POCA) inhibits carnitine palmityltransferase I and fatty acid oxidation. The effects of POCA on cardiac function and on tissue levels of carnitine and coenzyme A esters were studied in the isolated rat heart subjected to 90 minutes of ischemia with and without 15 minutes of reperfusion. The perfusion medium contained 1.2 mM palmitate and 5.5 mM glucose plus or minus 0.5 mM POCA. This compound prevented accumulation of long-chain acylcarnitine and coenzyme A esters during ischemia and significantly improved the recovery of cardiac output after ischemia and reperfusion. Short-chain acylcarnitine levels were increased during ischemia by POCA. No effects were noted on tissue ATP and lactate levels. POCA may protect the ischemic heart by preventing accumulation of these toxic metabolites and by stimulating glucose utilization during ischemia.

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Author notes

  1. D. J. Paulson

    Present address: Metabolic Research Laboratory, William S. Middleton Memorial Veterans Administration Hospital, 2500 Overlook Terrace, 53705, Madison, WI, (USA)

Authors and Affiliations

  1. Metabolic Research Laboratory, William S. Middleton Memorial Veterans Administration Hospital, 2500 Overlook Terrace, 53705, Madison, WI, (USA)

    J. J. Noonan, K. M. Ward, H. Stanley &  A. L. Shug

  2. Department of Neurology, University of Wisconsin-Madison, 53706, Madison, WI, (USA)

    J. J. Noonan, K. M. Ward, H. Stanley &  A. L. Shug

  3. Department of Physiology, Chicago College of Osteopathic Medicine, 60615, Chicago, IL

    D. J. Paulson

  4. Department of Pharmacological Sciences, University of Newcastle upon Tyne, NE1 7RU, Newcastle upon Tyne, UK

    A. Sherratt

Authors
  1. D. J. Paulson
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  2. J. J. Noonan
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  4. H. Stanley
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  5. A. Sherratt
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  6. A. L. Shug
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Additional information

Sodium 2-[5-(4-chlorophenyl)-pentyl]-oxirane-2-carboxylate

This work was supported by the Veterans Administration and NIH HL 17736

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Paulson, D.J., Noonan, J.J., Ward, K.M. et al. Effects of POCA on metabolism and function in the ischemic rat heart. Basic Res Cardiol 81, 180–187 (1986). https://doi.org/10.1007/BF01907382

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  • DOI: https://doi.org/10.1007/BF01907382

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