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PHB recovery by hypochlorite digestion of non-PHB biomass

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Biotechnology Techniques

Summary

Hypochlorite digestion of bacterial biomass from intracellular poly-β-hydroxybutyrate (PHB) has not been used on a large scale since it has been reported to severely degrade PHB. In this study, to minimize degradation, the initial biomass concentration, digestion time and pH of the hypochlorite solution were optimized. Consequently, PHB of 95% purity with a weight average molecular weight (MW) of 600,000 and a polydispersity index (PI) of 4.5 was recovered from biomass initially containing PHB with a MW of 1,200,000 and a PI of 3.

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Authors and Affiliations

  1. Chemical Engineering, Ecole Polytechnique de Montreal, Succursale “A”, C.P. 6079, H3C 3A7, Montreal, Canada

    E. Berger, B. A. Ramsay, J. A. Ramsay & C. Chavarie

  2. Institut für Biotechnologie, Technische Universtität, Schlögelgasse 9, A-8010, Graz, Austria

    G. Braunegg

Authors
  1. E. Berger
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  2. B. A. Ramsay
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  3. J. A. Ramsay
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  4. C. Chavarie
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  5. G. Braunegg
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Berger, E., Ramsay, B.A., Ramsay, J.A. et al. PHB recovery by hypochlorite digestion of non-PHB biomass. Biotechnol Tech 3, 227–232 (1989). https://doi.org/10.1007/BF01876053

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  • DOI: https://doi.org/10.1007/BF01876053

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