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Production of poly-β-hydroxybutyrate by activated sludge in sequencing batch reactor under aerobic conditions

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Abstract

In order to improve poly-β-hydroxybutyrate (PHB) production in activated sludge, the anaerobic/aerobic alternative operating sequencing batch reactor (SBR) process was applied in this paper to accumulate PHB. Effects of nutritional conditions and carbon concentration on PHB accumulation were studied. Results indicated that PHB accumulation reached the highest level and accounted for 11.2 % under anaerobic condition for phosphate limitation and 20.84 % under aerobic condition for nitrogen and phosphate limitation of mixed liquor suspended solid (MLSS), respectively. In addition, 4 g/L was proved to be the optimum carbon concentration in both anaerobic and aerobic experiments, and the PHB accumulation reached 17.1 % (anaerobic, phosphorus limitation) and 60.4 % (aerobic, nitrogen and phosphorus limitation) of MLSS, respectively. PHB could be successfully extracted with sodium hypochlorite and chloroform method from the activated sludge. In addition, the infrared spectrum showed that the PHB sample extracted was of high purity.

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Authors and Affiliations

  1. College of Life Sciences, Northeast Forestry University, Harbin, 150040, China

    Xiaoyan Li  (李晓岩), Zijing Jin, Yongyu Qian, Daizong Cui & Min Zhao  (赵敏)

  2. College of Marine Life Science, Ocean University of China, Qingdao, 266003, China

    Xiguang Chen

Authors
  1. Xiaoyan Li  (李晓岩)
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  2. Zijing Jin
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  3. Yongyu Qian
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  4. Daizong Cui
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  5. Xiguang Chen
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  6. Min Zhao  (赵敏)
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Corresponding author

Correspondence to Min Zhao  (赵敏).

Additional information

LI Xiaoyan and JIN Zijing equally contributed to this research as co-first authors

Funded by the Fundamental Research Funds for the Central Universities (No.2572014CA23), the National Natural Science Foundation of China (No. 51678120)

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Li, X., Jin, Z., Qian, Y. et al. Production of poly-β-hydroxybutyrate by activated sludge in sequencing batch reactor under aerobic conditions. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 32, 733–738 (2017). https://doi.org/10.1007/s11595-017-1660-4

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  • DOI: https://doi.org/10.1007/s11595-017-1660-4

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