Summary
An improved technique of protoplast regeneration and a general procedure of transfection and plasmid transformation are described inClostridium acetobutylicum. Frequencies of 104–105 transfectants/transformants per μg of DNA were easily achieved.
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Reysset, G., Hubert, J., Podvin, L. et al. Transfection and transformation ofClostridium acetobutylicum strain N1-4081 protoplasts. Biotechnol Tech 2, 199–204 (1988). https://doi.org/10.1007/BF01875765
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DOI: https://doi.org/10.1007/BF01875765