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Bifidobacterium Transformation

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Bifidobacteria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2278))

Abstract

The protocol presented in this chapter describes a generic method for electrotransformation of Bifidobacterium spp., outlining a technique that is ideal for conferring selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we are able to achieve transformation efficiencies up to 107 transformants/μg of DNA.

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Acknowledgments

This work was sponsored by Nutricia Research, Utrecht, The Netherlands. E.C.H., F.B., J.M., and D.v.S. are members of APC Microbiome Ireland, which is funded by Science Foundation Ireland (SFI) through the Irish Government’s National Development Plan (Grant Numbers SFI/12/RC/2273-P1 and SFI/12/RC/2273-P2).

Author information

Authors and Affiliations

  1. APC Microbiome Ireland, University College Cork, Cork, Ireland

    Emily C. Hoedt, Francesca Bottacini & John MacSharry

  2. Danone Nutricia Research, Utrecht, The Netherlands

    Roger S. Bongers & Jan Knol

  3. Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands

    Jan Knol

  4. School of Microbiology, University College Cork, Cork, Ireland

    John MacSharry

  5. School of Medicine, University College Cork, Cork, Ireland

    John MacSharry

  6. School of Microbiology & APC Microbiome Ireland, University College Cork, Cork, Ireland

    Douwe van Sinderen

Authors
  1. Emily C. Hoedt
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  2. Roger S. Bongers
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  3. Francesca Bottacini
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  4. Jan Knol
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  5. John MacSharry
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  6. Douwe van Sinderen
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Corresponding author

Correspondence to Douwe van Sinderen .

Editor information

Editors and Affiliations

  1. Microbiome Ireland, School of Microbiology & APC, University College Cork, Cork, Ireland

    Douwe van Sinderen

  2. Laboratory of Probiogenomics, Department of Chemistry, Life Sciences & Environmental Sustainability, University of Parma, Parma, Italy

    Marco Ventura

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Hoedt, E.C., Bongers, R.S., Bottacini, F., Knol, J., MacSharry, J., van Sinderen, D. (2021). Bifidobacterium Transformation. In: van Sinderen, D., Ventura, M. (eds) Bifidobacteria. Methods in Molecular Biology, vol 2278. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1274-3_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1274-3_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1273-6

  • Online ISBN: 978-1-0716-1274-3

  • eBook Packages: Springer Protocols

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