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Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells

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Summary

The calcium sensitivity of exocytosis from electroper-meabilized chromaffin cells is increased by activators of protein kinase C, such as TPA and certain phorbol esters, diacylglycerols, and mezerein. A range of putative inhibitors of protein kinase C block both the phorbol ester-sensitive component of secretion and also the underlying insensitive component. These inhibitors are also shown to inhibit medulla protein kinase C activity in vitro. The extent of secretion is reduced when electropermeabilized cells are exposed to Ca2+ levels much in excess of 50 μm. The onset of inhibition is faster than the relatively slow rate of Ca-dependent exocytosis and is insensitive to inhibitors of proteolysis. Adrenal medulla protein kinase C activity is also irreversibly inhibited by high Ca2+ concentrations. Both the secretory response and the protein kinase C activity in vitro have similar nucleotide and cation specificities. Although these data do not definitely establish an involvement of protein kinase C in exocytosis, none argue against it.

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Authors and Affiliations

  1. MRC Secretory Mechanism Group, Division of Biomedical Sciences, Kings College, WC2R2LS, London, England

    D. E. Knight, D. Sugden & P. F. Baker

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  1. D. E. Knight
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  2. D. Sugden
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  3. P. F. Baker
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Knight, D.E., Sugden, D. & Baker, P.F. Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells. J. Membrain Biol. 104, 21–34 (1988). https://doi.org/10.1007/BF01871899

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  • DOI: https://doi.org/10.1007/BF01871899

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