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Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells

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Exocytosis and Endocytosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2233))

Abstract

Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline.

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Author information

Authors and Affiliations

  1. Department of Neuroscience, University of Copenhagen, Copenhagen N, Denmark

    Sébastien Houy, Joana S. Martins & Jakob Balslev Sørensen

  2. Institute for Physiology, Otto-von-Guericke University, Magdeburg, Germany

    Ralf Mohrmann

Authors
  1. Sébastien Houy
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  2. Joana S. Martins
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  3. Ralf Mohrmann
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  4. Jakob Balslev Sørensen
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Corresponding author

Correspondence to Jakob Balslev Sørensen .

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Editors and Affiliations

  1. Department of Infection, Immunity & Inflammation, Institut Cochin (Institut National de la santé et de la recherche médicale, Centre National de la Recherche Scientifique and Université de Paris), Paris, France

    Florence Niedergang

  2. Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France

    Nicolas Vitale

  3. Centre National de la Recherche Scientifique, Institut des Neurosciences Cellulaires et Intégratives, Université de Strasbourg, Strasbourg, France

    Stéphane Gasman

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Houy, S., Martins, J.S., Mohrmann, R., Sørensen, J.B. (2021). Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells. In: Niedergang, F., Vitale, N., Gasman, S. (eds) Exocytosis and Endocytosis. Methods in Molecular Biology, vol 2233. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1044-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1044-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1043-5

  • Online ISBN: 978-1-0716-1044-2

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