Summary
Glucose uptake into plasma membrane vesicles from the maternal surface of the human placenta was measured with the Millipore filtration technique. Uptake ofd-glucose was dependent on the osmolarity of the incubation medium surrounding the vesicles. Uptake ofd-glucose exceeded that ofl-glucose. The uptake ofd-glucose was not enhanced by placing 100mm NaCl or NaSCN in the medium outside the vesicles (none inside) at the onset of uptake determinations.d-glucose transport was inhibited by cytochalasin B; phloretin, phlorizin, and 1-fluoro-2,4-dinitrobenzene.d-glucose uptake was inhibited by 2-deoxy-d-glucose, 3-O-methyl-d-glucose and to a lesser extent byd-galactose. It was not inhibited by α-methyl-d-glucoside. Cytochalasin B binding to the vesicles was 30% inhibited in the presence of 80mm d-glucose. The results indicate that the system for facilitated transport ofd-glucose at the maternal face of the placenta is distinctly different from that on the brush-border membrane of intestine or renal tubule and more closely resembles that of human erythrocyte.
Similar content being viewed by others
References
Bowers, G.N., McComb, R.B. 1966. A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.Clin. Chem. (N.Y.) 12:70
Carstensen, M., Leichtweiss, H.-P., Molsen, G., Schröder, H. 1977. Evidence for a specific transport ofd-hexoses across the human placentain vitro.Arch. Gynaekol. 222:187
Carter, J.R., Jr., Avruch, J., Martin, D.B. 1972. Glucose transport in plasma membrane vesicles from rat adipose tissue.J. Biol. Chem. 247:2682
Cordero, L., Jr., Yen, S.-Y., Grunt, J.A., Anderson, G.G. 1970. Hypertonic glucose infusion during labor.Am. J. Obstet. Gynecol. 107:295
Czech, M. 1976. Characterization of [3H] cytochalasin B binding to the fat cell plasma membrane.J. Biol. Chem. 251:2905
Hopfer, U. 1978. Transport in isolated plasma membranes.Am. J. Physiol. 234 (Renal Fluid Electrolyte Physiol. 3):F89
Hopfer, U., Nelson, K., Perrotto, J., Isselbacher, K.J. 1973. Glucose transport in isolated brush border membrane from rat small intestine.J. Biol. Chem. 248:25
Huggett, A.St.G., Warren, F.L., Warren, N.W. 1951. The origin of the blood fructose of the foetal sheep.J. Physiol. (London) 113:258
Hulstaert, C.E., Torringa, J.L., Koudstaal, J., Hardouk, M.J., Malenaar, I. 1973. The characterisitc distribution of alkaline phosphatase in the full-term human placenta.Gynecol. Invest. 4:24
Johnson, L.W., Smith, C.H. 1979. Hexose transport to the human fetus: Role of the syncytial microvillous membrane.Fed. Proc. 38:1188
Jung, C.Y., Rampal, A.L. 1977. Cytochalasin B binding sites and glucose transport carrier in human erythrocyte ghosts.J. Biol. Chem. 252:5456
Kinne, R., Murer, H., Kinne-Saffran, E., Thees, M., Sachs, G. 1975. Sugar transport by renal plasma membrane vesicles.J. Membrane Biol. 21:375
LeFevre, P.G. 1961. Sugar transport in the red blood cell: Structure-activity relationships in substrates and antagonists.Pharmacol. Rev. 13:39
Lin, S., Spudich, J.A. 1974. Biochemical studies on the mode of action of cytochalasin B.J. Biol. Chem. 249:5778
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurements with the Folin phenol reagent.J. Biol. Chem. 193:265
Murer, H., Hopfer, U. 1974. Demonstration of electrogenic Na+-dependentd-glucose transport in intestinal brush border membranes.Proc. Nat. Acad. Sci. (USA) 71:484
Oakley, N.W., Beard, R.W., Turner, R.C. 1972. Effect of sustained maternal hyperglycaemia on the fetus in normal and diabetic pregnancies.Brit. Med. J. 1:466
Rice, P.A., Nesbitt, R.E.L., Jr., Rourke, J.E. 1976.In vitro perfusion studies of the human placenta. V. Counter transport of glucose induced by an analog.Gynecol. Invest. 7:344
Rice, P.S., Rourke, J.E., Nesbitt, R.E.L., Jr. 1976.In vitro perfusion studies of the human placenta. IV. Some characteristics of the glucose transport system in the human placenta.Gynecol. Invest. 7:213
Rice, P.S., Rourke, J.E., Nesbitt, R.E.L., Jr. 1979.In vitro perfusion studies of the human placenta. III. Evidence against active glucose transport.Am. J. Obstet. Gynecol. 133:649
Smith, C.H., Nelson, D.M., King, B.F., Donohue, T.M., Ruzycki, S.M., Kelly, L.K. 1977. Characterization of a microvillous membrane preparation from human placental syncytiotrophoblast: A morphological biochemical and physiological study.Am. J. Obstet. Gynecol. 128:190
Smith, N.C., Brush, M.G., Luckett, S. 1975. Preparation of human placental villous surface membrane.Nature (London) 252:301
Taverna, R.D., Langdon, R.G. 1973. Reversible association of cytochalasin B with the human erythrocyte membrane.Biochim. Biophys. Acta 323:207
Turner, R.J., Silverman, M. 1977. Sugar uptake into brush border vesicles from normal human kidney.Proc. Nat. Acad. Sci. (USA) 74:2825
Wardzala, L.J., Cushman, S.W., Salans, L.B. 1978. Mechanism of insulin action on glucose transport in the isolated rat adipose cell.J. Biol. Chem. 253:8002
Widdas, W.F. 1952. Inability of diffusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer.J. Physiol. (London) 118:23
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bissonnette, J.M., Black, J.A., Wickham, W.K. et al. Glucose uptake into plasma membrane vesicles from the maternal surface of human placenta. J. Membrain Biol. 58, 75–80 (1981). https://doi.org/10.1007/BF01871036
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01871036