Summary
A Na/Ca exchange system has been described in the plasma membrane of several tissues and seems to regulate the concentration of calcium in cytosol. Replacement of extracellular Na by sucrose increases calcium uptake into and decreases calcium efflux from the cell, leading to an increase in cytosolic calcium. The effect of an increase in cytosolic calcium mediated by the Na/Ca exchange system on H+ and Na transport in the turtle and toad bladder was investigated by replacing serosal Na isosmotically by sucrose or choline. Replacement of serosal by sucrose was associated with a significant inhibition of H+ secretion or Na transport which was reversible by addition of NaCl. Replacement of mucosal Na by sucrose failed to alter H+ secretion. Removal of serosal Na was associated with a significant increase in45Ca uptake which could be blocked by pretreatment with lanthanum chloride. Pretreatment with lanthanum chloride blunted the inhibitory effect of replacement of serosal Na by sucrose on H+ and Na transport, thus suggesting that the increase in calcium uptake and the inhibition of transport are causally related. Under anaerobic conditions the rate of H+ or Na transport are linked to the rate of lactate production. The inhibition of Na or H+ transport by removal of serosal Na was accompanied by a proportional decrease in lactate production, thus suggesting that an increase in cytosolic calcium does not inhibit transport by uncoupling glycolysis from transport. Replacement of serosal Na by sucrose did not alter the force of the H+ or Na pump but led to an increase in resistance of the active pathway of H+ and Na transport. The inhibition of Na transport by replacement of serosal Na with sucrose could be reversed by addition of amphotericin B, an agent which increases luminal permeability to Na, thus suggesting that decreased Na entry across the apical membrane is the mechanism responsible for the inhibition of Na transport. The results of the present studies strongly suggest that an increase in cytosolic calcium through the serosal Na/Ca exchange system inhibits H+ and Na transport in the turtle and toad bladder probably by increasing the resistance of the luminal membrane.
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References
Aguilera, J., Kirk, K.L., DiBona, G.F. 1978. Effect of magnesium on sodium transport in toad urinary bladder.Am. J. Physiol. 234:F192-F198
Arruda, J.A.L. 1979. Calcium inhibits urinary acidification: Effects of ionophore A23187 on the turtle bladder.Pfluegers Arch. 381:107–111
Arruda, J.A.L., Dytko, G., Mola, R. 1979. Effect of calcium and magnesium on transport processes by the turtle bladder.Arch. Int. Pharmacodyn. Ther. 240:27–34
Arruda, J.A.L., Dytko, G., Mola, R., Kurtzman, N.A. 1980. On the mechanism of lithium induced distal renal tubular acidosis in the turtle bladder.Kidney Int. 17:196–203
Arruda, J.A.L., Sabatini, S. 1980a. Cholinergic inhibition of urinary acidification in the turtle bladder.Kidney Int. 17:622–630
Arruda, J.A.L., Sabatini, S. 1980b. Cholinergic modulation of water transport in the toad bladder.Am. J. Physiol. 239:F154-F159
Arruda, J.A.L., Sabatini, S. 1980c. Effect of quinidine on sodium, hydrogen ion and water transport in the turtle and toad bladders.J. Membrane Biol. 55:141–147
Arruda, J.A.L., Sabatini, S., Westenfelder, C. 1981. Vanadate inhibits H+ secretion in the turtle bladder at the level of the pump.Kidney Int. 20:772–779
Baker, P.F. 1978. The regulation of intracellular Ca in giant axons of loligo and myxicola.Ann. N.Y. Acad. Sci. 307:250–268
Blaustein, M.P. 1974. The interrelationship between sodium and calcium fluxes across cell membranes.Rev. Physiol. Biochem. Pharmacol. 70:33–82
Borle, A. 1973. Calcium metabolism at the cellular level.Fed. Proc. 32:1944–1950
Chase, H.S., Al-Awqati, Q. 1981. Regulation of sodium permeability of the luminal border of the toad bladder by intracellular sodium and calcium; Role of sodium-calcium exchange in the basolateral membrane.J. Gen. Physiol. 77:693–712
Gatzy, J.T., Reuss, L., Finn, A.L. 1979. Amphotericin B and K transport across excised toad urinary bladder.Am. J. Physiol. 237:F145-F156
Grinstein, S., Erlij, D. 1978. Intracellular calcium and the regulation of sodium transport in the frog skin.Proc. R. Soc. London B 202:353–360
Hardy M.A. 1977. Intracellular calcium as a modulator of transepithelial permeability to water in frog urinary bladder.J. Cell Biol. 76:787–791
Hardy, M.A., Balsam, P., Bourgoignie, J.J. 1979. Reversible inhibition by lanthanum of the hydrosmotic response to serosal hypertonicity in toad urinary bladder.J. Membrane Biol. 48:13–19
Hardy, M.A. 1979. Roles of Na+ and Ca+ in the inhibition by low pH of the hydrosmotic response to serosal hypertonicity in toad bladder.Biochim. Biophys. Acta 552:169–177
Isaacson, L.C. 1977. Resolution of parameters in the equivalent electrical circuit of the sodium transport mechanism across toad skin.J. Membrane Biol. 30:301–317
Klahr, S., Bricker, N.N. 1965. Energetics of anaerobic sodium transport by the fresh water turtle bladder.J. Gen. Physiol. 48:571–580
Lichtenstein, N.S., Leaf, A. 1965. Effect of amphotericin B on the permeability of the toad bladder.J. Clin. Invest. 44:1328–1342
Macchia, D.D. 1977. Characterization of theI–V Relationships of the Toad Urinary Bladder, Toad Colon, Frog Skin, and Turtle Urinary Bladder. Ph.D. Thesis. University of Illinois, Urbana, Illinois
Macey, R.I., Koblick, D.C. 1963. Effects of choline and other quaternary ammonium compounds on Na movements in frog skin.Am. J. Physiol. 205:1063–1066
Macknight, A., DiBona, D., Leaf, A. 1980. Sodium transport across toad urinary bladder: A model “tight” epithelium.Physiol. Rev. 60:616–715
Miyamoto, H., Racker, E. 1980. Solubilization and partial purification of the Ca2+/Na+ antiporter from the plasma membrane of bovine heart.J. Biol. Chem. 255:2656–2658
Mullins, L.J. 1981. The Mechanism of Ca Transport in Squid Axons in Membrane Transport Processes. D.C. Tosteson, editor. Vol. 2, pp. 371–381. Y.A. Ovchinniko and R. Latore, editors. Raven Press, New York
Nakagawa, K., Klahr, S., Bricker, N.S. 1967. Sodium transport by the isolated epithelium of the urinary bladder of the fresh water turtle.Am. J. Physiol. 213:1565–1569
Navarte, J., Finn, A.C. 1980. Microelectrode studies in toad urinary bladder epithelium: Effects of Na concentration changes in the mucosal solution on equivalent electromotive forces.J. Gen. Physiol. 75:323–344
Rasmussen, H., Goodman, D.B.P. 1977. Relationship between calcium and cyclic nucleotides in cell activation.Physiol. Rev. 57:421–509
Reeves, J.P., Sutko, J.L. 1979. Sodium-calcium ion exchange in cardiac membrane vesicles.Proc. Natl. Acad. Sci. USA 76:590–594
Reuss, L., Finn, A.L. 1974. Passive electric properties of toad urinary epithelium: Intracellular coupling and transepithelial cellular and shunt conductances.J. Gen. Physiol. 64:1–25
Schilb, T.P., Brodsky, W.A. 1966. Acidification of mucosal fluid by transport of bicarbonate ion in turtle bladders.Am. J. Physiol. 210:997–1008
Steinmetz, P.R., Husted, F.F., Mueller, A., Beauwens, R. 1981. Coupling between H+ transport and anaerobic glycolysis in turtle urinary bladder: Effect of inhibitors of H+ ATPase.J. Membrane Biol. 59:27–34
Taylor, A., Windhager, E.E. 1979. Possible role of cytosolic calcium and Na-Ca exchange in regulation of transepithelial transport.Am. J. Physiol. 236:F505-F512
Van Breemen, C., Aaronson, P., Loutzenhiser, R. 1979. Sodium-calcium interaction in mammalian smooth muscle.Pharmacol. Rev. 30:167–197
Wiesmann, W., Sinha, S., Klahr, S. 1977. Effects of ionophore A23187 on baseline and vasopressin stimulated sodium transport in the toad bladder.J. Clin. Invest. 59:418–425
Wiesmann, W., Sinha, S., Klahr, S. 1978. Cholinergic agents inhibit sodium transport across isolated toad bladder.Am. J. Physiol. 235:F564-F569
Yonath, J., Civan, M. 1971. Determination of the driving force of Na pump in the toad bladder by means of vasopressin.J. Membrane Biol. 5:366–385
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Arruda, J.A.L., Sabatini, S. & Westenfelder, C. Serosal Na/Ca exchange and H+ and Na+ transport by the turtle and toad bladders. J. Membrain Biol. 70, 135–146 (1982). https://doi.org/10.1007/BF01870223
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DOI: https://doi.org/10.1007/BF01870223