Skip to main content
SpringerLink
Log in

Interaction of bilayers with basic polypeptides

  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Summary

The interaction of bilayers formed from glyceryl monooleate+oleic acid with basic polypeptides was studied. Copolymers of lysine with different amino acids interact with the bilayers reducing their resistance and increasing their capacitance.

The largest effect was observed with the copolymerl-lysine/l-phenylalanine (1.4∶1) which decreases the specific resistance by more than 3 orders of magnitude. The smallest effects on the resistance were obtained with the very hydrophilic copolymer ofl-lysine/l-serine (1.1∶1). The contribution of the double-layer potential to the measured transmembrane potentials of the charged bilayers is considered and calculated for the case of the membrane containing dissociable components in the absence of interacting copolymers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Cherry, R. J., Berger, K. U., Chapman, D. 1971. Interaction of erythrocyte apoprotein with bimolecular lipid membranes.Biochem. Biophys. Res. Commun. 44:644.

    PubMed  Google Scholar 

  2. Davies, J. T., Rideal, E. K. 1963. Interfacial Phenomena, p. 237. Academic Press Inc., New York.

    Google Scholar 

  3. Fettiplace, R., Andrews, D. M., Haydon, D. A. 1971. The thickness, composition and structure of some lipid bilayers and natural membranes.J. Membrane Biol. 5:277.

    Google Scholar 

  4. Giannoni, G., Padden, F. J., Roe, R. J. 1971. A lamellar complex of lecithin and poly-l-tyrosine.Biophys. J. 11:1018.

    PubMed  Google Scholar 

  5. Grahame, D. C. 1947. The electrical double layer and the theory of electrocapillarity.Chem. Rev. 41:441.

    Article  Google Scholar 

  6. Hammes, G. G., Schullery, S. E. 1970. Structure of macromolecular aggregates. II. Construction of model membranes from phospholipids and polypeptides.Biochemistry 9:2555.

    PubMed  Google Scholar 

  7. Hanai, T., Haydon, D. A., Taylor, J. 1964. An investigation by electrical methods of lecithin-in-hydrocarbon films in aqueous solutions.Proc. Roy. Soc. (London) A 281:377.

    Google Scholar 

  8. Harned, H. S., Owen, B. B. 1963. The Physical Chemistry of Electrolytic Solutions, p. 224, Reinhold, New York.

    Google Scholar 

  9. Jain, M. K., Strickholm, A., Cordes, E. H. 1969. Reconstitution of an ATP-mediated active transport system across black lipid membranes.Nature 222:871.

    PubMed  Google Scholar 

  10. Katchalsky, A., Shavit, N., Eisenberg, H. 1954. Dissociation of weak polymeric acids and bases.J. Polymer Sci. 13:69.

    Google Scholar 

  11. Kimelberg, H. K., Papahadjopoulos, D. 1971. Interactions of basic proteins with phospholipid membranes.J. Biol. Chem. 246:1142.

    PubMed  Google Scholar 

  12. MacDonald, R. C., Bangham, A. D. 1972. Comparison of double layer potentials in lipid monolayers and lipid bilayer membranes.J. Membrane Biol. 7:29.

    Google Scholar 

  13. Montal, M. 1972. Lipid-polypeptide interactions in bilayer lipid membranes.J. Membrane Biol. 7:245.

    Google Scholar 

  14. Mueller, P., Rudin, D. O., Tien, H. T., Wescott, W. C. 1962. Reconstitution of excitable cell membrane structure in vitro.Circulation 26:1167.

    Google Scholar 

  15. Redwood, W. R., Müldner, H., Thompson, T. E. 1969. Interaction of a bacterial adenosine triphosphatase with phospholipid bilayers.Proc. Nat. Acad. Sci. 64:989.

    PubMed  Google Scholar 

  16. Stein, J. M., Tourtellotte, M. E., Reinert, J. C., McElhaney, R. W., Rader, R. L. 1969. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane.Proc. Nat. Acad. Sci. 63:104.

    PubMed  Google Scholar 

  17. Stein, W. D. 1967. The Movement of Molecules across Cell Membranes, p. 13. Academic Press Inc., New York.

    Google Scholar 

  18. Steinemann, A., Läuger, P. 1971. Interaction of cytochromec with phospholipid monolayers and bilayer membranes.J. Membrane Biol. 4:74.

    Google Scholar 

  19. Sweet, C., Zull, J. E. 1969. Activation of glucose diffusion from egg lecithin liquid crystals by serum albumin.Biochim. Biophys. Acta 173:94.

    PubMed  Google Scholar 

  20. Thompson, T. E., Henn, F. A. 1970. Experimental phospholipid model membranes.In: Membranes of Mitochondria and Chloroplasts. E. Racker, editor. p. 1. Reinhold, New York.

    Google Scholar 

  21. Vetter, K. J. 1967. Electrochemical Kinetics, pp. 28, 49. Academic Press Inc., New York.

    Google Scholar 

  22. Wilkins, M. H. F., Blaurock, A. E., Engelman, D. M. 1971. Bilayer structure in membranes.Nature, New Biol. 230:72.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Polymer Department, The Weizmann Institute of Science, Rehovot, Israel

    D. Bach & I. R. Miller

Authors
  1. D. Bach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. R. Miller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bach, D., Miller, I.R. Interaction of bilayers with basic polypeptides. J. Membrain Biol. 11, 237–254 (1973). https://doi.org/10.1007/BF01869825

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01869825

Keywords

Search

Navigation