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Thyroidal enhancement of rat myocardial Na,K-ATPase: Preferential expression of α2 activity and mRNA abundance

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Summary

In hypothyroid rat myocardium, the low-ouabain-sensitivity Na,K-ATPase activity had aK i =10−4 m and accounted for ∼95% of the enzyme activity, while the high-ouabain-sensitivity activity contributed ∼5% to the total activity, with aK i =3×107 m. mRNAα1 was 7.2- and 5.5-fold more abundant than mRNAα2 and mRNAβ, respectively, in hypothyroid ventricles while mRNAα3 was undetectable. Administration of T3 increased total Na,K-ATPase activity 1.6-fold; the low-ouabain-sensitivity activity increased 1.5-fold while high-ouabain-sensitivity activity was stimulated 3.2-fold. T3 increased the number of high-affinity ouabain-binding sites 2.9-fold with no change inK d (∼2×10−7 m). The abundances of mRNAα1, mRNAα2, and mRNAβ (per unit RNA) following T3 treatment increased 3.6-, 10.6-, and 12.7-fold, respectively. The larger increments in subunit mRNA abundances than in Na,K-ATPase activity suggests the involvement of translational and/or post-translational regulatory steps in Na,K-ATPase biogenesis in response to T3. It is concluded that T3 enhances myocardial Na,K-ATPase subunit mRNA abundances and Na,K-ATPase activity, and that the expression of the high- and low-ouabain-sensitivity activities are probably a reflection of the abundances of the α2 and α1 isoforms, respectively. The physiological role played by the β subunit remains uncertain.

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  1. Gregory G. Gick

    Present address: Department of Biochemistry, State University of New York Health Science Center at Brooklyn, 11203, Brooklyn, New York

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, 10032, New York, New York

    Gregory G. Gick, Jivan Melikian & Faramarz Ismail-Beigi

  2. Department of Medicine, College of Physicians and Surgeons, columbia University, 10032, New York, New York

    Gregory G. Gick, Jivan Melikian & Faramarz Ismail-Beigi

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Gick, G.G., Melikian, J. & Ismail-Beigi, F. Thyroidal enhancement of rat myocardial Na,K-ATPase: Preferential expression of α2 activity and mRNA abundance. J. Membrain Biol. 115, 273–282 (1990). https://doi.org/10.1007/BF01868642

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