Summary
An attempt is made to correlate structure and transport function in the embryonic chorioallantoic membrane. The fine structure of the endoderm and ectoderm in the membrane was examined with particular attention given to the morphological changes that occur when transport is established,in vivo. Two distinctive cells, a granule-rich cell and a mitochondria-rich cell, appear in the endoderm at the time allantoic fluid sodium, chloride and water reabsorption commences. These are indistinguishable from the cells described in toad bladder epithelium. It is suggested that the granule-rich cell is responsible for bulk water movement and the mitochondria-rich cell is specifically engaged in active sodium transport. In the ectoderm, two distinctive cell types are also found to be associated with the onset of active calcium transport. These are referred to as the capillary-covering cell and the villus-cavity cell. The preponderate capillary-covering cell is most likely responsible for transcellular calcium transport. It is postulated that the function of the villus-cavity cell is to secrete hydrogen ions which are necessary, along with carbonic anhydrase, to mobilize Ca++ from the insoluble calcium carbonate of the eggshell.
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References
Anderson, E., Harvey, W. R. 1966. Active transport byCecropia midgut. II. Fine structure of the midgut epithelium.J. Cell Biol. 31:107.
Borysko, E., Bang, F. B. 1953. The fine structure of the chorioallantoic membrane of the normal chick embryo.Johns Hopkins Hosp. Bull. 92:257.
Choi, J. K. 1963. The fine structure of the urinary bladder of the toad,Bufo marinus.J. Cell Biol. 16:53.
Coleman, J. R., DeWitt, S. M., Batt, P., Terepka, A. R. 1970. Electron probe analysis of calcium distribution during active transport in chick chorioallantoic membrane.Exp. Cell Res. 63:216.
Dawes, C., Simkiss, K. 1969. The acid-base status of the blood of the developing chick embryo.J. Exp. Biol. 50:79.
DiBona, D. R., Civan, M. M., Leaf, A. 1969. The cellular specificity of the effect of vasopressin on toad urinary bladder.J. Membrane Biol. 1:79.
Farquhar, M. G., Palade, G. E. 1963. Junctional complexes in various epithelia.J. Cell Biol. 17:375.
Flumerfelt, B. A., Gibson, M. A. 1969. The histology and histochemistry of the developing chorioallantoic membrane in the chick (Gallus domesticus).Canad. J. Zool. 47:323.
Ganote, C. E., Beaver, D. L., Moses, H. L. 1964. Ultrastructure of the chick chorioallantoic membrane and its reaction to inoculation trauma.Lab. Invest. 13:1575.
Garrison, J. C., Terepka, A. R. 1972. Calcium-stimulated respiration and active calcium transport in isolated chick chorioallantoic membrane.J. Membrane Biol. 7:128.
Goodenough, D. A., Revel, J. P. 1970. A fine structural analysis of intercellular junctions in the mouse liver.J. Cell Biol. 45:272.
Hamilton, H. L. 1952. Lillie's Development of the Chick. Holt, Rinehart and Winston, Inc., New York.
Ito, S., Winchester, R. J. 1963. The fine structure of the gastric mucosa in the bat.J. Cell Biol. 16:541.
Johnston, P. M., Comar, C. L. 1955. Distribution and contribution of calcium from the albumin, yolk and shell to developing chick embryo.Amer. J. Physiol. 183:365.
Leeson, T. S., Leeson, C. R. 1963. The chorio-allantois of the chick. Light and electron microscopic observations at various times of incubation.J. Anat. 97:585.
Moriarty, C. M., Terepka, A. R. 1969. Calcium transport by the isolated chick chorio-allantoic membrane.Arch. Biochem. Biophys. 135:160.
Palade, G. E. 1952. A study of fixation for electron microscopy.J. Exp. Med. 95:285.
Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy.J. Cell Biol. 17:208.
Romanoff, A. L. 1961. The Avian Embryo. MacMillan, New York.
Sabatini, D. D., Bensch, K., Barnett, R. J. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.J. Cell Biol. 17:19.
Stewart, M. E., Terepka, A. R. 1969. Transport functions of the chick chorioallantoic membrane. I. Normal histology and evidence for active electrolyte transport from the allantoic fluid,in vivo.Exp. Cell Res. 58:93.
Sweeny, P. R., Bather, R. 1968. An electron microscopic study of the chorioallantoic membrane following infection with Rous sarcoma virus.J. Cell Biol. 36:299.
Terepka, A. R. 1963. Structure and calcification in avian egg shell.Exp. Cell Res. 30:171.
Terepka, A. R. 1963. Organic-inorganic interrelationships in avian egg shell.Exp. Cell Res. 30:183.
Terepka, A. R., Coleman, J. R., Garrison, J. C., Spataro, R. F. 1971. Active transcellular transport of calcium by embryonic chick chorioallantoic membrane.In: Cellular Mechanisms for Calcium Transfer and Homeostasis. G. Nichols and R. H. Wasserman, editors. p. 371. Academic Press Inc., New York.
Terepka, A. R., Stewart, M. E., Merkel, N. 1969. Transport functions of the chick chorio-allantoic membrane. II. Active calcium transport,in vitro.Exp. Cell Res. 58:107.
Venable, J. H., Coggeshall, R. J. 1966. A simplified lead citrate stain for use in electron microscopy.J. Cell Biol. 25:407.
Watson, M. L. 1958. Staining of tissue sections for electron microscopy with heavy metals.J. Biophys. Biochem. Cytol. 4:475.
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Coleman, J.R., Terepka, A.R. Fine structural changes associated with the onset of calcium, sodium and water transport by the chick chorioallantoic membrane. J. Membrain Biol. 7, 111–127 (1972). https://doi.org/10.1007/BF01867911
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DOI: https://doi.org/10.1007/BF01867911