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Der Einfluß von Glukokortikoiden auf die physiologische Zellerneuerung der Magen- und Darmschleimhaut

The influence of glucocorticoids on the physiological cell renewal of gastric and intestinal mucosa

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Research in Experimental Medicine

Summary

The influence of Methylprednisolone (1.2 mg/kg body weight daily for 14 days) on the physiological cell renewal of gastric and intestinal mucosa was examined autoradiographically by determination of the3H-labelling index and the cell cycle of the regenerating epithelial cells. Macroscopical or microscopical changes of the gastric or intestinal mucosa could not be observed. The labelling index was not significantly altered compared to the controls. Against this the determination of the cell cycle showed a clear inhibition of epithelial cell proliferation in all parts of the gastrointestinal tract with a prolongation of the postmitotic phase, a small inhibition of the DNA synthesis and a corresponding prolongation of the mean generation time.

Zusammenfassung

Der Einfluß von Methylprednisolon (1,2 mg/kg KG/d über 14 Tage) auf die physiologische Zellerneuerung der Magen- und Darmschleimhaut wurde an männlichen NMRI-Mäusen durch Bestimmung des3H-Markierungsindex sowie des Zellzyklus der regenerierenden Epithelzellen autoradiographisch untersucht. Makroskopische oder mikroskopische Veränderungen der Magen- oder Darmschleimhaut waren nicht nachweisbar. Während der prozentuale Anteil der DNS synthetisierenden Epithelzellen keine signifikante Abweichung von der Norm aufwies, fand sich bei der Zellzyklusanalyse am Epithel des gesamten Magen-Darmkanals eine deutliche Wachstumshemmung mit einer Verlängerung der postmitotischen Ruhephase bis auf das Mehrfache der Norm, geringer Verlängerung der DNS-Synthesephase und entsprechender Vergrößerung der mittleren Generationszeit.

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  1. Klinik und Poliklinik für Allgemeinchirurgie, Universität Göttingen, Goßlerstraße 10, D-3400, Göttingen, Bundesrepublik Deutschland

    H. J. Castrup, A. Reiß & K. Neumann

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  1. H. J. Castrup
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  2. A. Reiß
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  3. K. Neumann
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Castrup, H.J., Reiß, A. & Neumann, K. Der Einfluß von Glukokortikoiden auf die physiologische Zellerneuerung der Magen- und Darmschleimhaut. Res. Exp. Med. 174, 159–167 (1979). https://doi.org/10.1007/BF01851328

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  • DOI: https://doi.org/10.1007/BF01851328

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