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Sensitivity of CFU-E to exogenous erythropoietin in benzene-treated mice

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Summary

Normal as well as hypertransfused BDF1 mice were exposed to 300 ppm of benzene, 6 h/day, 5 days per week for 2, 4, and 6 weeks respectively. Erythroid-committed hematopoietic progenitor cells CFU-E were determined in the bone marrow, and59Fe incorporation was measured in the peripheral blood 48 h after its injection, as an indicator of active erythroid cell production. CFU-E numbers were reduced in benzene-exposed mice at all intervals, as was59Fe incorporation in the peripheral blood after 2 weeks of exposure. In hypertransfused mice the CFU-E suppression, caused primarily by the hypertransfusion, was aggravated by benzene. After injection of 1 IU Ep 4 days before killing the CFU-E numbers and the59Fe incorporation increased in controls as well as in benzene-exposed animals, but the difference persisted. After nine consecutive Ep injections the difference concerning the femur disappeared after 2 and 6 weeks of benzene exposure but was still present in the peripheral blood. These results suggest that chronic benzene exposure has a negative effect on early erythroid-committed, Ep-responsive hematopoietic cells.

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Authors and Affiliations

  1. Institute of Occupational and Social Medicine, University of Ulm, Albert-Einstein-Allee 11, W-7900, Ulm, FRG

    E. Barthel & H. J. Seidel

  2. Department of Pathophysiology, First Medical Faculty, Charles University, Prague, Czechoslovakia

    E. Necas

Authors
  1. E. Necas
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  2. E. Barthel
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  3. H. J. Seidel
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Necas, E., Barthel, E. & Seidel, H.J. Sensitivity of CFU-E to exogenous erythropoietin in benzene-treated mice. Ann Hematol 64, 12–15 (1992). https://doi.org/10.1007/BF01811465

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  • DOI: https://doi.org/10.1007/BF01811465

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