Abstract
The relative distributions of 480DR2-relatedDR, DQ haplotypes have been determined in Australian Aborigines, Papua New Guinean Highlanders, coastal Melanesians, Micronesians, Polynesians, Javanese, and Southern and Northern Chinese. Using sequence-specific oligonucleotides (SSOs) for hybridization of polymerase chain reaction (PCR) products from DRBI,DRBS,DQA1, andDQBI genes, 15 differentDR2-related haplotypes were identified. The predominantDR2 haplotype in Oceania involved a novel combination ofDRBI * 1502,DRB5 * 0101 alleles; this haplotype occurred sporadically in Java, but not in China. In Southern China, the most frequent DR2 haplotype involved the unusual arrangementDRB1 * 1602,DRB5 * 0101; alternatively,DRB1 * 1602 was associated with a newDRB5 SSO pattern. This study has important implications for molecular HLA-typing protocols that assume particularDRB1 DRB5 orDR,DQ linkage relationships. Further, the novelDRBI,DRB5 haplotype in Oceania suggests that the mixed lymphocyte culture (MLC) determinants Dw2 and Dw12 are discriminated by codon 86 at theDRBI locus.
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Gao, X., Serjeantson, S.W. Heterogeneity inHLA-DR2-relatedDR, DQ haplotypes in eight populations of Asia-Oceania. Immunogenetics 34, 401–408 (1991). https://doi.org/10.1007/BF01787491
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DOI: https://doi.org/10.1007/BF01787491