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Changes in the collagenolytic activity released by primary VX-2 carcinoma cultures as a function of tumor growth

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Summary

Serum-free media of minced tissue cultures of VX-2 rabbit carcinoma contained a specific collagenolytic activity capable of releasing soluble radioactive peptides from [14C]-labeled collagen fibrils. It was also capable of reducing the viscosity of acid-soluble collagen solutions by cleaving the tropocollagen (TC) molecules primarily at one site to TCA (75%) and TCB (25%) fragments. Three chromatographic fractions were separated by gel filtration: F1, (MW 85-110,000) present in larger amounts in early cultures of younger tumor tissue; F2, (MW 35-40,000) the major component with maximum production in the day 3 media of younger and advanced tumor tissues; F3, (MW 18-22,000) the minor component. Early cultures of younger tumor tissue contained a latent collagenase and were subject to trypsin activation suggesting the presence of inactive enzyme precursors or an enzyme-inhibitor complex.

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Authors and Affiliations

  1. The Departments of Biochemistry and Microbiology, University of Tennesse Center for the Health Sciences, 38163, Memphis, Tennessee, USA

    Mustafa Kh. Dabbous, Camelia Sobhy, Audrey N. Roberts &  Burcharda Brinkley

Authors
  1. Mustafa Kh. Dabbous
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  2. Camelia Sobhy
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  3. Audrey N. Roberts
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  4. Burcharda Brinkley
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Additional information

This work was supported by USPHS NCI Grant CA 15677.

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Dabbous, M.K., Sobhy, C., Roberts, A.N. et al. Changes in the collagenolytic activity released by primary VX-2 carcinoma cultures as a function of tumor growth. Mol Cell Biochem 16, 37–42 (1977). https://doi.org/10.1007/BF01769837

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  • DOI: https://doi.org/10.1007/BF01769837

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