Abstract
A consensus sequence for the P protein gene of the RSN-2 strain of respiratory syncytial (RS) virus was obtained by PCR amplification of cDNA obtained by reverse transcription. This established that the extent of sequence variation between two P genes of strains of antigenic subtype B is similar to that among A strains, confirming the conservation of P genes within subtypes and the divergence of the two antigenic subtypes of RS virus.
The P protein of RS virus exhibits anomalous electrophoretic mobility with respect to its molecular weight. In vitro transcription and translation of RSN-2 strain cDNA possessing single point mutations revealed that substitutions involving charged amino acids in the carboxy-terminal region had a marked effect on the electrophoretic mobility of the P protein.
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Caravokyri, C., Pringle, C.R. Effect of changes in the nucleotide sequence of the P gene of respiratory syncytial virus on the electrophoretic mobility of the P protein. Virus Genes 6, 53–62 (1992). https://doi.org/10.1007/BF01703757
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DOI: https://doi.org/10.1007/BF01703757