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Impact of mutations in nucleoprotein on replication of influenza virus A/Hong Kong/1/68/162/35 reassortants at different temperatures

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Abstract

The nucleoprotein (NP) of influenza virus is a multifunctional RNA binding protein. The role of NP in the adaptation of influenza viruses to a host has been experimentally proved. Ambiguous data are available on the role of nucleoprotein in the attenuation of influenza A viruses, which is characterized by ability to replicate at low temperature (26°C) and inability to replicate at high temperature (39°C). Influenza virus donor strain A/Hong Kong/1/68/162/35 (H3N2), adapted to growth at low temperature, differs from the wild type virus by 14 amino acid mutations in the internal and non-structural proteins. Two mutations occurred in the NP: Gly102Arg and Glu292Gly. We have obtained viruses with point reverse-mutations in these positions and compared their replication at different temperatures by measuring infectious activity in chicken embryos. It has been shown that reverse mutation Gly292Glu in the NP reduced virus ability to replicate at low temperature, the introduction of the second reverse mutation Arg102Gly completely abolished virus cold adaptation.

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Abbreviations

att:

attenuated (phenotype)

ca:

cold adapted (phenotype)

NP:

nucleoprotein

RCT37, RCT26 :

reproductive capacity at different temperatures

ts:

temperature sensitive (phenotype)

CE:

embryonated chicken eggs

EID50 :

50% embryo infectious dose.

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Authors and Affiliations

  1. Research Institute of Influenza WHO National Influenza Centre of Russia, St. Petersburg, 197376, Russia

    A. A. Pulkina, M. V. Sergeeva, S. V. Petrov, A. V. Fadeev, A. B. Komissarov, E. A. Romanovskaya-Romanko, M. V. Potapchuk & L. M. Tsybalova

  2. Peter the Great St. Petersburg Polytechnic University, Saint-Petersburg, 195251, Russia

    A. A. Pulkina

Authors
  1. A. A. Pulkina
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  2. M. V. Sergeeva
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  3. S. V. Petrov
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  4. A. V. Fadeev
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  5. A. B. Komissarov
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  6. E. A. Romanovskaya-Romanko
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  7. M. V. Potapchuk
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  8. L. M. Tsybalova
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Corresponding author

Correspondence to M. V. Sergeeva.

Additional information

Original Russian Text © A.A. Pulkina, M.V. Sergeeva, S.V. Petrov, A.V. Fadeev, A.B. Komissarov, E.A. Romanovskaya-Romanko, M.V. Potapchuk, L.M. Tsybalova, 2017, published in Molekulyarnaya Biologiya, 2017, Vol. 51, No. 2, pp. 378–383.

The article was translated by the authors.

The authors contributed equally.

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Pulkina, A.A., Sergeeva, M.V., Petrov, S.V. et al. Impact of mutations in nucleoprotein on replication of influenza virus A/Hong Kong/1/68/162/35 reassortants at different temperatures. Mol Biol 51, 333–338 (2017). https://doi.org/10.1134/S0026893317010149

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  • DOI: https://doi.org/10.1134/S0026893317010149

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