Abstract
Somatic embryogenesis was achieved in callus cultures derived from immature cotyledonary explants ofHardwickia binata Roxb., a multipurpose leguminous tree, on semisolid modified Murashige and Skoog's (mMS) medium containing 2900 mg/l potassium nitrate (KNO3) supplemented with 4.64 µM kinetin (Kn) and 5.37µM a-naphthaleneacetic acid (NAA). Somatic embryos proliferated rapidly after transfer to MS basal medium supplemented with 2052.6 µM L-glutamine and 0.084 µM gibberellic acid (GA3). Maturation of somatic embryos was achieved on half-strength MS basal medium supplemented with 1.23 µM IBA and 2% (w/v) sucrose. Histological studies confirmed different developmental stages of somatic embryogenesis inHardwickia binata.
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Abbreviations
- BA:
-
N6-benzyladenine
- Kn:
-
kinetin
- NAA:
-
a-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- GA3 :
-
gibberellic acid
- MS:
-
Murashige and Skoog (1962) medium
- mMS:
-
modified Murashige and Skoog (1962) medium
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Communicated by G. C. Phillips
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Das, A.B., Rout, G.R. & Das, P. In vitro somatic embryogenesis from callus culture of the timber yielding treeHardwickia binata Roxb.. Plant Cell Reports 15, 147–149 (1995). https://doi.org/10.1007/BF01690273
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DOI: https://doi.org/10.1007/BF01690273