Abstract
The autotrophic CO2 fixation pathway inAcidianus brierleyi, a facultatively anaerobic thermoacidophilic archaebacterium, was investigated by measuring enzymatic activities from autotrophic, mixotrophic, and heterotrophic cultures. Contrary to the published report that the reductive tricarboxylic acid cycle operates inA. brierleyi, the enzymatic activity of ATP:citrate lyase, the key enzyme of the cycle, was not detected. Instead, activities of acetyl-CoA carboxylase and propionyl-CoA carboxylase, key enzymes of the 3-hydroxypropionate cycle, were detected only whenA. brierleyi was growing autotrophically. We conclude that a modified 3-hydroxypropionate pathway operates inA. brierleyi.
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Abbreviations
- TCA :
-
tricarboxylic acid
- BV :
-
Benzyl viologen
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Ishii, M., Miyake, T., Satoh, T. et al. Autotrophic carbon dioxide fixation inAcidianus brierleyi . Arch. Microbiol. 166, 368–371 (1996). https://doi.org/10.1007/BF01682981
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DOI: https://doi.org/10.1007/BF01682981