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Immunisation trials with live attenuated cytomegalovirus TOWNE 125

Immunisierungsversuche mit lebenden attenuierten Zytomegalieviren TOWNE 125

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Summary

Trials with live cytomegalovirus (TOWNE 125 strain), which was attenuated by 125 passages exclusively on WI-38 cells, were done in adult volunteers. No virus take occured after oral/nasal application. When 103 TCD50 was given intramuscularly an IgG antibody response was detected at four weeks in all of ten volunteers tested by immunofluorescence; an IgM response was found in seven. Only mild local side-effects and relative lymphocytosis were observed. No virus excretion was found. Many questions remain to be answered by further trials before a cytomegalovirus vaccine can be given to adolescent girls.

Zusammenfassung

Bei erwachsenen Freiwilligen wurden Immunisierungsversuche mit lebenden Zytomegalieviren, welche durch 125 Passagen ausschließlich auf WI-38 Zellen attenuiert worden waren (TOWNE 125 Stamm), durchgeführt. Orale/nasale Applikation induzierte keine Antikörperbildung. Fluoreszierende IgG-Antikörper konnten bei allen zehn sero-negativen Probanden, welche die attenuierten Zytomegalieviren in einer Dosis von 103 TCD50 intramuskulär erhielten, vier Wochen nach Immunisierung gefunden werden. Zum Auftreten von IgM-Antikörpern kam es bei sieben der zehn Sero-Negativen. Klinisch traten nur geringfügige Lokalreaktionen und eine relative Lymphozytose auf. Es konnte keine Virusausscheidung nachgewiesen werden. Verschiedene bis jetzt noch ungeklärte Fragen müssen zuerst durch weitere „Impfversuche“ an Freiwilligen geklärt werden, bevor an eine generelle Durchimpfung weiblicher Adoleszenten gegen Zytomegalie gedacht werden kann.

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Authors and Affiliations

  1. Department of Microbiology and Serology, University Children's Hospital, Römergasse 8, CH-4000, Basel 5, Switzerland

    M. Just, A. Buergin-Wolff, G. Emoedi & R. Hernandez

Authors
  1. M. Just
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  2. A. Buergin-Wolff
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  3. G. Emoedi
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  4. R. Hernandez
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Additional information

This work was partly supported by a grant from Schweizer Nationalfonds (4.124–0.74).

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Just, M., Buergin-Wolff, A., Emoedi, G. et al. Immunisation trials with live attenuated cytomegalovirus TOWNE 125. Infection 3, 111–114 (1975). https://doi.org/10.1007/BF01641052

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  • DOI: https://doi.org/10.1007/BF01641052

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