Summary
The multi-locus systems (MLS) 33.15 and 33.6 (Jeffreys et al 1985a) and the single-locus systems (SLS) MS 1, MS 8, MS 31 and MS 43 (Wong et al. 1987) were investigated. The number of bands and the rate of band sharing were determined for both multi-locus systems and compared to the results of an English survey. Additionally 73 families were investigated using the multi-locus probes and the results compared to those obtained for the traditional blood grouping systems. The results were in full agreement so that no evidence of new mutations could be found. The fragment distribution was calculated for each of the 4 single-locus systems and compared to the values reported in an English survey (Smith et al. 1990b). Distinct discrepancies were seen in the systems MS 1 and MS 8. Family analyses were carried out for the 4 single-locus systems and compared to English data (Jeffreys et al. 1988) to look for any indications of possible new mutations. Only one isolated exclusion could be demonstrated with MS 1.
Zusammenfassung
Untersucht wurden die von Jeffreys et al. (1985a) entdeckten Multi-Locus-Systeme (MLS) 33.15 and 33.6 sowie die Single-Locus-Systeme (SLS) MS 1, MS 8, MS 31 and MS 43 (Wong et al. 1987). Für die beiden Multi-Locus-Systeme wurden die Bandenzahlen und die Band-Sharing-Raten ermittelt und mit einer englischen Stichprobe verglichen. Zusätzlich erfolgte für 73 Familien ein Vergleich der MLS-Untersuchungsergebnisse mit konventionellen Gutachten. Die Ergebnisse stimmten in allen Fällen überein; es fand sich kein Hinweis auf Neumutationen. Für die 4 Single-Locus-Systeme wurde die jeweilige Fragmentverteilung dargestellt und mit den Werten einer englischen Stichprobe (Smith et al. 1990b) verglichen. Auffällige Abweichungen zeigten sich bei den Systemen MS 1 and MS 8. Desweiteren erfolgte eine Gegenüberstellung der errechneten Hete rozygoten-Frequenzen beider Stichproben (MünsterEngland). Um Hinweise auf mögliche Neumutationen zu erhalten, wurden für die 4 SLS Familienanalysen durchgeführt und mit englischen Daten (Jeffreys et al. 1988) verglichen. Lediglich in MS 1 ließ sich ein isolierter Ausschluß zum Eventualvater feststellen.
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References
Baird M, Balazs I, Giusti A, Miyazaki L, Nicholas L, Wexler K, Kanter E, Glassberg J, Allen F, Rubinstein P, Sussman L (1986) Allele frequency distribution of two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity. Am J Hum Genet 39:489–501
Brinkmann B, Biitler R, Lincoln R, Mayr WR, Rossi U (1989) Recommendations of the Society of Forensic Haemogenetics concerning DNA polymorphisms. Forensic Sci Int 43:109–111
Davis LG, Dibner MD, Battey JF (1986) Basic methods in molecular biology. Elsevier, New York
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA “fingerprints/rd. Nature 318:577–579
Gjertson DW, Mickey MR, Hopfield J, Takenouchi T, Terasaki PI (1988) Calculation of probability of paternity using DNA sequences. Am J Hum Genet 43:860–869
Jeffreys AJ, Wilson V, Thein SL (1985a) Hypervariable “minisatellite/rd regions in human DNA. Nature 314:67–73
Jeffreys AJ, Wilson V, Thein SL (1985b) Individual-specific “fingerprints/rd of human DNA. Nature 316:76–79
Jeffreys AJ, Royle NJ, Wilson V, Wong Z (1988) Spontaneous mutation rates to new length alleles at tandem-repetitive hypervariable loci in human DNA. Nature 332:278–281
Kömpf J, Driesel AJ (1989) DNA-Polymorphismen in Blutgruppengutachten. Biotech-Forum 6:182–184
Lander ES (1989a) DNA Fingerprinting on trial. Nature 339: 501–505
Lander ES (1989b) Population genetic considerations in the forensic use of DNA typing. In: Ballantyne J, Sensabaugh G, Wittkowski J (eds) Banbury Report 32: DNA technology and forensic Science. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New York
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A new laboratory manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New York
Morris JW, Sanda AI, Glassberg J (1990) Biostatistical evaluation of evidence from continous allele frequency distribution. Deoxyribonucleic acid (DNA) probes in reference to disputed paternity and identity. J Forensic Sci 34: 1311–1317
Smith JC, Newton CR, Alves A, Anwar R, Jenner D, Markham AF (1990a) Highly polymorphic minisatellite DNA probes. Further evaluation for individual identification and paternity testing. J Forensic Sci Soc 30:3–18
Smith JC, Anwar R, Riley J, Jenner D, Markham AF, Jeffreys AJ (1990b) Highly polymorphic minisatellite sequences: allele frequencies and mutation rates for five locus-specific probes in a Caucasian population. J Forensic Sci Soc 30:19–32
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Wong Z, Wilson V, Patel I, Povey S, Jeffreys AJ (1987) Characterisation of a panel of highly variable minisatellites cloned from human DNA. Ann Hum Genet 51:269–288
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Brinkmann, B., Rand, S. & Wiegand, P. Population and family data of RFLP's using selected single- and multi-locus systems. Int J Leg Med 104, 81–86 (1991). https://doi.org/10.1007/BF01626036
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DOI: https://doi.org/10.1007/BF01626036