Skip to main content
SpringerLink
Log in

Growth suppression of transformed cells by a human placental extract not related to transforming growth factor Β

  • Original Papers
  • Experimental Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Summary

We have examined whether human placental extracts contain tumour-growth-inhibitory factors. One fraction (EAP) from such extracts inhibited growth, in soft agar, ofHa-ras-transformed BALB/c 3T3 cells and human squamous lung carcinoma A-2182 cells. However, this fraction had no effect on the anchorage-dependent growth of these cells, although there was a slight mitogenic activity on nontransformed cells. These data together with those on plating efficiency indicated no significant cytotoxicity of EAP on transformed cell lines. Although this fraction contained transforming growth factorΒ (TGFΒ), this cannot account for its inhibitory activity, since (a) pure TGFΒ does not inhibit anchoragedependent growth of Ha-ras-transformed BALB/c 3T3 cells, (b) EAP retains its inhibitory activity in the presence of antibodies against TGFΒ and (c) the inhibitory activity did not copurify with TGFΒ. Partial characterization of our inhibitory factor suggests that the inhibitory factor is a new tumour-growth-inhibitory factor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

SDS:

sodium dodecyl sulphate

TGFα orΒ :

transforming growth factorα orΒ

TIF1 or 2:

transformed inhibitory factor 1 or 2

A-2182:

human squamous lung carcinoma cell line

BALB/c 3T3 1-1:

mouse fibroblast cell line, clone A31 1-1

BALB/c 3T3 Ha-ras, BALB/c 3T3 1-1:

cells transformed by Ha-ras oncogene

CHO:

Chinese hamster ovary; MEM, minimum essential medium

References

  • Aklyama SK, Larjava H, Yamada KM (1990) Differences in the biosynthesis and localization of the fibronectin receptor in the normal and transformed cultured human cells. Cancer Res 50:1601–1607

    Google Scholar 

  • Bischof P, Klopper A (1985) Proteins of the placenta. Biochemistry, biology and clinical application. Karger, Basel

    Google Scholar 

  • Derynck R, Jarrett JA, Chen EY, Eaton DH, Bell JR, Assoian RK, Roberts AB, Sporn MB, Goeddel DV (1985) Human transforming growth factor-Β complementary DNA sequence and expression in normal and transformed cells. Nature 316:701–705

    Google Scholar 

  • Enomoto T and Yamasaki H (1984) Lack of intercellular communication between chemically transformed and surrounding untransformed BALB/c 3T3 cells. Cancer Res 44:5200

    Google Scholar 

  • Foon KA (1989) Biological response modifiers: the new immunotherapy. Cancer Res 49:1621–1639

    Google Scholar 

  • Friend S, Bernard R, Rogelj S, Weinberg R, Rapaport J, Albert D, Dryja T (1986) A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma. Nature 323:643–646

    Google Scholar 

  • Frolik CA, Dart LL, Meyers CA, Smith DM, Sporn MB (1983) Purification and initial characterization of typeΒ transforming growth factor from human placenta. Proc Natl Acad Sci USA 80:3676–3680

    Google Scholar 

  • Fryling CM, Iwata KK, Johnson PA, Knott WB, Todaro GJ (1985) Two distinct tumor cell growth-inhibiting factors from a human rhabdomyosarcoma cell line. Cancer Res 45:2695–2699

    Google Scholar 

  • Fryling C, Dombalagian M, Burgess W, Hollander N, Schreiber AB, Haimovich J (1989) Purification and characterization of tumor inhibitory factor-2: its identity to interleukin 1. Cancer Res 49:3333–3337

    Google Scholar 

  • Gessler M, Poustka A, Cavenee W, Neves R, Orkin S, Bruns G (1990) Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping. Nature 343:774–778

    Google Scholar 

  • Giancotti FG, Ruoslahti E (1990) Elevated levels of theα 5 Β 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Cell 60:849–859

    Google Scholar 

  • Heino J, Ignotz RA, Hemler ME, Crousse C, Massague J (1989) Regulation of cell adhesion receptor by transforming growth factor-Β. J Biol Chem 264:380–388

    Google Scholar 

  • Hsuan JJ (1989) Transforming growth factorΒ. Br Med Bull 45:425–437

    Google Scholar 

  • Ignotz RA, Heino J, Massague J (1989) Regulation of cell adhesion receptor by transforming growth factor-Β. J Biol Chem 264:389–392

    Google Scholar 

  • Iwata KK, Fryling CM, Knott WB, Todaro GJ (1985) Isolation of tumor cell growth-inhibiting factors from a human rhabdomyosarcoma cell line. Cancer Res 45:2689–2694

    Google Scholar 

  • Kahn P, Shin S (1979) Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability. J Cell Biol 82:1–16

    Google Scholar 

  • Kakunaga T, Yamasaki H (1985) Transformation assay of established cell lines: mechanisms and application. (IARC Scientific Publications 67). International Agency for Research on Cancer, Lyon

    Google Scholar 

  • Klein G (1990) Tumor suppressor genes. Dekker, New York

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 282:619–621

    Google Scholar 

  • Moses HL, Coffer RJ, Leof EB, Lyons RM, Keski-Oja J (1987) Transforming growth factor-Β regulation of cell proliferation. J Cell Physiol 5:1–7

    Google Scholar 

  • Plantefaber L, Hynes RO (1989) Changes in integrin receptors on oncogenically transformed cells. Cell 56:281–290

    Google Scholar 

  • Roberts AB, Sporn MB (1988) Transforming growth factor-Β. Adv Cancer Res 51:107–145

    Google Scholar 

  • Ruoslahti E, Pierschbacher M (1987) New perspectives in cell adhesion: RGD and integrins. Nature 238:491–497

    Google Scholar 

  • Sager R (1985) Genetic suppression of tumor formation. Adv Cancer Res 44:43–68

    Google Scholar 

  • Santos E, Martin-Zanca D, Reddy ER, Pierotti MA, Della Porta G, Barbacid M (1984) Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient. Science, 223:661–664

    Google Scholar 

  • Sporn MB, Robert AB, Wakefield LM, Assoian RK (1986) Transforming growth factor-Β: biological function and chemical structure. Science 233:532–534

    Google Scholar 

  • Towbin HH, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Yamasaki H, Katoh F (1988) Further evidence for the involvement of gap junctional intercellular communication and maintenance of transformed foci in BALB/c 3T3 cells. Cancer Res 48:3490

    Google Scholar 

  • Yamasaki H, Hollstein M, Mesnil M, Martel N, Aguelon AM (1987) Selective lack of intercellular communication between transformed and nontransformed cells as a common property of chemical and oncogene transformation of BALB/c 3T3 cells. Cancer Res 47:5658–5664

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. International Agency for Research on Cancer, 150 cours Albert Thomas, F-69372, Lyon Cedex 08

    J. L. Klein, E. Hamel & H. Yamasaki

  2. Institut Merieux, Marcy-l'Etoile, France

    J. L. Tayot

Authors
  1. J. L. Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Hamel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. L. Tayot
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Yamasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was supported by a training grant from Groupement d'Etude et de Recherche sur le Placenta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klein, J.L., Hamel, E., Tayot, J.L. et al. Growth suppression of transformed cells by a human placental extract not related to transforming growth factor Β. J Cancer Res Clin Oncol 117, 192–196 (1991). https://doi.org/10.1007/BF01625424

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01625424

Key words

Search

Navigation