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The effect of casein phosphopeptides on calcium utilization in young ovariectomized rats

Einfluß von Kasein-Phosphopeptiden auf die Kalziumverwertung bei jungen ovarektomierten Ratten

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Summary

The effect of casein phosphopeptides (CPPs) on Ca utilization in ovariectomized (OVX) rats was studied. A mixture of CPPs corresponding to the amino acid sequences 1–25 and 1–28 in the β-casein was isolated from the tryptic digest of β-casein (βCPP).

After being fed a low Ca diet for 30 days, OVX rats were fed experimental diets of which the Ca level was 0.1 %, 0.3 % or 0.5 % with or without 0.15 % βCPP for 28 days. During days 1–3 of the Ca refeeding period, rats fed βCPP with 0.5 % Ca showed a higher Ca absorption than control rats not supplemented with βCPP.

During days 7–9 and 26–28, there were no significant differences in Ca and P balances between the βCPP group and the control group for each dietary Ca level. Femoral Ca and P contents from rats fed βCPP tended to be higher than those from control rats. These results suggest that βCPP supplementation could have an effect on Ca absorption at a certain degree of Ca deficiency.

Zusammenfassung

Gegenstand der Untersuchung war der Einfluß von Kasein-Phosphopeptiden (CPPs) auf die Kalziumverwertung bei ovarektomierten Ratten (OVX). Aus dem tryptischen Extrakt von β-Kasein (βCPP) wurde eine den Aminosäuresequenzen 1–25 und 1–28 im β-Kasein entsprechende CPP-Mischung isoliert. Nach Füttern mit einer Ca-armen Diät über 30 Tage wurden die OVX-Ratten 28 Tage mit Versuchsdiäten mit Ca-Niveaus von 0,1 %, 0,3 % bzw. 0,5 % sowie mit bzw. ohne 0,15 % βCPP gefüttert. An den Tagen 1–3 der Ca-Ergänzungsperiode zeigten Ratten, an die βCPP mit 0,5 % Ca verfüttert wurde, eine höhere Ca-Absorption als ohne βCPP-Zusatz ernährte Kontrollratten. An den Tagen 7–9 und 26–28 war zwischen der βCPP-Gruppe und der Kontrollgruppe für das jeweilige Ca-Niveau kein sinnvoller Unterschied im Ca- und P-Haushalt feststellbar. Die femoralen Ca- und P-Gehalte von mit βCPP gefütterten Ratten tendierten zu höheren Werten als bei den Kontrollratten. Diese Ergebnisse deuten darauf hin, daß die βCPP-Ergänzungen bei einem gewissen Grad von Ca-Mangel die Ca-Absorption beeinflussen könnten.

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Abbreviations

CPP:

casein phosphopeptide

OVX:

ovariectomized

FER:

feed efficiency ratio

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Authors and Affiliations

  1. Central Research Institute, Meiji Milk Products Co., Ltd., Japan

    H. Tsuchita, I. Sekiguchi & T. Kuwata

  2. Department of Food and Nutrition, Japan Women's University, Japan

    C. Igarashi & I. Ezawa

Authors
  1. H. Tsuchita
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  2. I. Sekiguchi
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  3. T. Kuwata
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  4. C. Igarashi
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  5. I. Ezawa
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Tsuchita, H., Sekiguchi, I., Kuwata, T. et al. The effect of casein phosphopeptides on calcium utilization in young ovariectomized rats. Z Ernährungswiss 32, 121–130 (1993). https://doi.org/10.1007/BF01614755

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