Abstract
The origin of the biosynthetic pathways for the branched-chain amino acids cannot be understood in terms of the backwards development of the present acetolactate pathway because it contains unstable intermediates. We propose that the first biosynthesis of the branched-chain amino acids was by the reductive carboxylation of short branched chain fatty acids giving keto acids which were then transaminated. Similar reaction sequences mediated by nonspecific enzymes would produce serine and threonine from the abundant prebiotic compounds glycolic and lactic acids. The aromatic amino acids may also have first been synthesized in this way, e.g. tryptophan from indole acetic acid. The next step would have been the biosynthesis of leucine from α-ketoisovaleric acid. The acetolactate pathway developed subsequently. The first version of the Krebs cycle, which was used for amino acid biosynthesis, would have been assembled by making use of the reductive carboxylation and leucine biosynthesis enzymes, and completed with the development of a single new enzyme, succinate dehydrogenase. This evolutionary scheme suggests that there may be limitations to inferring the origins of metabolism by a simple back extrapolation of current pathways.
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References
Allison, M. J.: 1978,Appl. Environ. Microbiol. 35, 872–877.
Allison, M. J. and Peel, J. L.: 1971,Biochem. J. 121, 431–437.
Bada, J. L. and Miller, S. L.: 1969,J. Am. Chem. Soc. 91, 3948–3949.
Belfaiza, J., Parsot, C, Martel, A., Bouthier de la Tour, C, Margarita, D., Cohen, G. N. and Saint-Girons, I.: 1986,Proc. Natl. Acad. Sci. U.S.A. 83, 867–871.
Breslow, R. and McNelis, E.: 1959,J. Am. Chem. Soc. 81, 3080–3082.
Chang, Y. and Cronan, J. E.: 1988,J. Bacteriol. 170, 3937–3945.
Cronin, J. R. and Pizzarello, S.: 1983,Adv. Space Res. 3 (9), 5–18.
Doctor, V. M. and Oró, J.: 1969,Biochem. J. 112. 691–697.
Eggerer, H., Stadtman, E. R., and Poston, J. M.: 1962,Arch. Biochem, Biophys. 98, 432–443.
Erickson, L. E. and Alberty, R. A.: 1959,J. Phys. Chem. 63, 705–709.
Friedmann, N. and Miller, S. L.: 1969,Science 166, 766–767.
Friedmann, N., Haverland, W. J., and Miller, S. L.: 1971, in Buvet, R. and Ponnamperuma, C. (Eds.),Chemical Evolution and the Origin of Life, North-Holland Publ. Co. Amsterdam, pp. 123–135.
Hegeman, G. D. and Rosenberg, S. L.: 1970,Ann. Rev. Microbiol. 24, 429–462.
Hill, R. K., Sawada, S. and Arfin, S. M.: 1979,Bioorg. Chem. 8, 175–189.
Horowitz, N. H.: 1945,Proc. Natl. Acad. Sci. U.S.A. 31, 153–157.
Horowitz, N. H.: 1965, in Bryson, V. and Vogel, H. J. (Eds.),Evolving Genes and Proteins, Academic Press, New York, pp. 15–23.
Hünig, S. and Schaller, R.: 1982,Angew. Chem. Int. Ed. Engl. 21, 36–49.
Jensen, R. A.: 1976,Ann. Rev. Microbiol. 30, 409–425.
Lawless, J. G. and Yuen, G. U.: 1979,Nature 282, 396–398.
Metzler, D. E. Ikawa, M., and Snell, E. E.: 1954,J. Am. Chem. Soc. 76, 648–652.
Miller, S. L.: 1957,Biochim. Biophys. Acta 23, 480–489.
Parsot, C: 1986,EMBO Journal 5, 3013–3019.
Peltzer, E. T. and Bada, J. L.: 1978.:Nature 272, 443–444.
Peltzer, E. T. Bada, J. L., Schlesinger, G., and Miller, S. L.: 1984,Adv. Space Res. 4 (12), 69–74.
Ring, D., Wolman, Y., Friedmann, N., and Miller, S. L.: 1972,Proc. Natl. Acad. Sci. U.S.A. 69, 765–768.
Robinson, I. M. and Allison, M. J.: 1969,J. Bacteriol. 97, 1220–1226.
Rozelle, L. T. and Alberty, R. A.: 1957,J. Phys, Chem. 61, 1637–1640.
Sauer, F. D., Erfle, J. D., and Mahadevan, S.: 1975,Biochem. J. 150, 357–372.
Shapiro, R.: 1988,Origins of Life 18, 71–85.
Shen, C, Yang, L. Miller, S. L., and Oro, J.: 1990,J. Mol. Evol. 31, 167–174.
Shen, C, Yang, L., Miller, S. L., and Oró, J.: 1987,Origins of Life 17, 295–305.
Tanaka, N. and Johnson, M. J.: 1971,J. Bacteriol. 108, 1107–1111.
Umbarger, H. E.: 1987, in Neidhardt, F. C, Ingraham, J. L., Low, K. B., Magasanik, B., Schaechter, M. and Umbarger, H. E. (Eds.),Escherichia coli and Salmonella typhimurium; Cellular and Molecular Biology, American Society for Microbiology, Washington, D. C. 1, 352–367.
Van Trump, J. E. and Miller, S. L.: 1972,Science 178, 859–860.
Wächtershäuser, C.: 1988,System. Appl. Microbiol. 10, 207–210.
Warren, W. A.: 1971,Arch. Biochem. Biophys. 143, 212–217.
Weber, A. L. and Miller, S. L.: 1981,J. Mol. Evol. 17, 273–284.
Xing, R. and Whitman, W. B.: 1991,J. Bacteriol. 173, 2086–2092.
Yčas, M.: 1974,J. Theor. Biol. 44, 145–160.
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Keefe, A.D., Lazcano, A. & Miller, S.L. Evolution of the biosynthesis of the branched-chain amino acids. Origins Life Evol Biosphere 25, 99–110 (1995). https://doi.org/10.1007/BF01581576
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DOI: https://doi.org/10.1007/BF01581576