Abstract
Epifluorescence microscopy was used to examine hindgut contents ofZootermopsis angusticollis (Hagen) termites for the presence of methanogenic bacteria, which can be identified on the basis of the fluorescence of the novel cofactors F420 and F350. Small, autofluorescent, rod-shaped bacteria of theMethanobrevibacter sp. morphotype were observed associated with three flagellates tentatively identified asTrichomitopsis termopsidis (Cleveland),Tricercomitus termopsidis Kirby andHexamastix termopsidis Kirby. Methanogens were not observed associated with any other protozoal morphotypes and were not numerous in the free-living state inZ. angusticollis hindgut fluid. Electron micrographs of thin sections of hindgut protozoa suggest methanogens are endosymbionts in the small trichomonad protozoa. Our observations are consistent with the finding of Odelson and Breznak that methane is a minor endproduct of the metabolism of termite gut microbiota.
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Lee, M.J., Schreurs, P.J., Messer, A.C. et al. Association of methanogenic bacteria with flagellated protozoa from a termite hindgut. Current Microbiology 15, 337–341 (1987). https://doi.org/10.1007/BF01577591
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DOI: https://doi.org/10.1007/BF01577591