Abstract
The mixed hemadsorption hybrid antibody (MHA.HA) test was applied successfully to the detection of antigens on the surface of testicular cells separated into sub-populations by velocity sedimentation at unit gravity in the “staput” apparatus. Normal serum from mice of all strains tested, both male and female, was found to contain a natural autoantibody that reacts with testicular cells of all mice tested, but not with sperm or other cells. This autoantibody is detectable at an age of 4–6 weeks in females, and reaches a plateau at about 10 weeks of age. The corresponding antigen is denoted TCDA, because it is evidently a Testicular Cell Differentiation Antigen. Microscopy of the cells forming rosettes in the MHA.HA test confirmed that the TCDA+ cells belong to the gametogenetic series. Because females as well as males produce the autoantibody we presume that TCDA is also present on female gametic cells although it was not feasible to test this adequately. The anti-TCDA autoantibody is not related to the natural autoantibody against sperm, which according to MHA.HA test occurs in the serum of males but not of virgin females.
Similar content being viewed by others
Abbreviations
- MHA.HA:
-
mixed hemadsorption hybrid antibody
- TC:
-
testicular cells
- TCDA:
-
testicular cells differentiation antigen
- BALB:
-
BALB/c
- BSA:
-
bovine serum albumin
- Sp:
-
sperm
- SpA:
-
sperm antigen
- Ig:
-
immunoglobulin
- PBS:
-
phosphate-buffered saline
- NMS:
-
normal mouse serum
- FBS:
-
fetal bovine serum
- SRBC:
-
sheep red blood cells
References
Bennett, D.: The T-locus of the mouse.Cell 6:441–454, 1975
Bennett, D., Goldberg, E., Dunn, L.C., and Boyse, E.A.: Serological detection of a cell-surface antigen specified by the T (Brachyury) mutant gene in the house mouse.Proc. Natl. Acad. Sci. U.S.A. 69:2076–2080, 1972
Boyse, E.A., Bressler, E., Iritani, C.A., and Lardis, M.P.: CytotoxicγM antibody in mouse alloantisera.Transplantation 9:339–340, 1970
Boyse, E.A. and Old, L.J.: Some aspects of normal and abnormal cell surface geneticsAnnu. Rev. Genet. 3:269–290, 1969
Boyse, E.A. and Old, L.J.: The immunogenetics of differentiation in the mouseHarvey Lectures, in press, 1976
Edelman, G.N. and Millette, C.F.: Molecular probes of spermatozoan structures.Proc. Natl. Acad. Sci. U.S.A. 68:2436–2440, 1971
Goldberg, E.H., Aoki, T., Boyse, E.A., and Bennett, D.: Detection of H-2 antigens on mouse spermatozoa by cytotoxicity test.Nature 228:570–572, 1970
Goldberg, E.H., Boyse, E.A., Bennett, D., Scheid, M., and Carswell, E.A.: Serological demonstration of H-Y (male) antigen on mouse sperm.Nature 232:478–480, 1971
Hämmerling, G.J., Mauve, G., Goldberg, E., and McDevitt, H.O.: Tissue distribution of Ia antigens: Ia on spermatozoa, macrophages and epidermal cells.Immunogenetics 1:428–437, 1975
Hämmerling, U., Stackpole, C.W., and Koo, G.C.: Hybrid antibodies for labeling cell surface antigens.Meth. Cancer Res. IX:255–282, 1973
Lerner, R.A., Wilson, C.B., Del Villano, B.C., McConahey, P.J., and Dixon, F.J.: Endogeneous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycoprotein, gp70.J. Exp. Med. 143:151–166, 1976
Meistrich, M.L., Bruce, W.R., and Clermont, Y.: Cellular composition of fractions of mouse testis cells following velocity sedimentation separation.Exp. Cell Res. 79:213–227, 1973
Menge, A.C. and Fuller, B.: Testis antigens of man and some other primates.Fertil. Steril. 26:473–479, 1975
Obata, Y., Ikeda, H., Stockert, E., and Boyse, E.A.: Relation of the GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.J. Exp. Med. 141:188–197, 1975
Radu, I. and Voisin, G.A.: Ontogenesis of spermatozoa autoantigens in guinea pigs.Differentiation 3:107–114, 1975
Wachtel, S.S., Koo, G.C., Zuckerman, E.E., Hämmerling, U., Scheid, M.P., and Boyse, E.A.: Serological cross reactivity between H-Y (male) antigens of mouse and man.Proc. Natl. Acad. Sci. U.S.A. 71:1215–1218, 1974
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koo, G.C., Mittl, L.R. & Boyse, E.A. Antibody to testicular cells in the serum of normal mice. Immunogenetics 4, 213–219 (1977). https://doi.org/10.1007/BF01575661
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01575661