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A barrier covering lignified cell walls of barley straw that restricts access by rumen microorganisms

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Abstract

In barley straw, all stem tissues except the chlorenchyma are lignified. Electronmicroscopic investigations employing staining for cell-wall constituents and replica techniques revealed the presence of a tertiary wall covering the secondary wall and a warty layer deposited on the tertiary wall. The tertiary wall was composed of cellulose fibrils orientated in all directions, which were embedded in matrix material. The warty layer was comprised of granules and globules that were in many instances associated with a thin, flat, continuous layer. Though the tertiary wall could be degraded, the warty layer was resistant to degradation by rumen microorganisms. Only where the warty layer was mechanically disrupted could underlying cell-wall material be degraded. Bacteria could also burrow beneath these layers from exposed cell walls at the cut edges of the plant material. The warty layer forms a barrier to cell-wall-degrading bacteria and limits their colonization of straw stem.

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Authors and Affiliations

  1. Department of Plant Cytology and Morphology, Agricultural University, Arboretumlaan 4, 6703 BD, Wageningen, The Netherlands

    Ferdinand M. Engels

  2. Hannah Research Institute, Ayr, Scotland

    Richard E. Brice

Authors
  1. Ferdinand M. Engels
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  2. Richard E. Brice
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Engels, F.M., Brice, R.E. A barrier covering lignified cell walls of barley straw that restricts access by rumen microorganisms. Current Microbiology 12, 217–223 (1985). https://doi.org/10.1007/BF01573334

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