Abstract
Antisera (B10.129×A)F1 anti-P and (B10×A)F1 anti-B10.P contain antibodies that define, in the PVP hemagglutination test, an antigen originally described as G or H-2.7. Of the independentH-2 haplotypes, the H-2.7 antigen is present inf, j, k, p, ands. In addition, the antisera also contain a weak cytotoxic antibody, distinct from anti-H-2.7. The cytotoxic antibody reacts with antigens controlled by theK orI regions. The hemagglutinating H-2.7 antibody does not have cytotoxic activity. The genetic determinant coding for antigen H-2.7 can be mapped into the chromosomal segment between theS andD regions. The H-2.7 antigen thus serves as a marker for a new region of theH-2 complex. The locus coding for antigen H-2.7 is designatedH-2 G and the correspondingH-2 regionG. The H-2.7 antigen has a tissue distribution distinct from that of the H-2 antigens controlled by theK orD regions. So far it could be detected primarily on erythrocytes.
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Abbreviations
- ACT:
-
ammonium chloride Tris
- BSA:
-
bovine serum albumin
- HBSS:
-
Hanks' balanced salt solution
- PBS:
-
phosphate buffered saline
- PVP:
-
polyvinylpyrrolidone
References
Amos, D.B., Bashir, H. Boyle, W., MacQueen, M., and Tiilikainen, A.: A simple microcytotoxicity test.Transplantation 7:220–222, 1969
Cudkowicz, G. and Lotzová, E.: Hemopoietic cell-defined components of the major histocompatibility complex of mice. Identification of responsive and unresponsive recipients for bone marrow transplants.Transplant. Proc. 5:1399–1405, 1973
Dorf, M.E., Plate, J.M.D., Stimpfling, J.H., and Benacerraf, B.: Characterization of immune response and mixed lymphocyte reactions in selected intra-H-2 recombinant strains.J. Immunol. 114: 602–605, 1975
Gelfand, M.C., Sachs, D.H., Lieberman, R., and Paul, W.E.: Ontogeny of B lymphocytes. III.H-2 linkage of a gene controlling the rate of appearance of complement receptor lymphocytes.J. Exp. Med. 139: 1142–1153, 1974
Hoecker, G., Pizarro, O., and Ramos, A.: Some new antigens and histocompatibility factors in the mouse.Transplant. Bull. 6:407–411, 1959
Klein, J.: The H-2 system: Past and present.Transplant. Proc. 5:11–21, 1973
Klein, J. and Shreffler, D.C.: TheH-2 model for major histocompatibility systems.Transplant. Rev. 6:3–29, 1971
Klein, J., Bach, F.H., Festenstein, H., McDevitt, H.O., Shreffler, D.C., Snell, G.D., and Stimpfling, J.H.: Genetic nomenclature for theH-2 complex of the mouse.Immunogenetics 1:184–188, 1974
Mühlbock, O. and Dux, A.: Histocompatibility genes (theH-2 complex) and susceptibility to mammary tumor virus in mice.J. Natl. Cancer Inst. 53:993–996, 1974
Shreffler, D.C. and Snell, G.D.: The distribution of thirteen H-2 alloantigenic specificities among the products of eighteen H-2 alleles.Transplantation 8:435–450, 1969
Snell, G.D., Cherry, M., and Démant, P.:H-2: Its structure and similarity to HL-A.Transplant. Rev. 15:3–25, 1973
Stimpfling, J.H.: The use of PVP as a developing agent in mouse hemagglutination tests.Transplant. Bull. 27:109–111, 1961
Stimpfling, J.H.: Presentation at theH-2 Workshop, Bar Harbor, Main, 1973
Widmer, M.B., Peck, A.B., and Bach, F.H.: Genetic mapping ofH-2 LD loci.Transplant. Proc. 5:1501–1505, 1973
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Klein, J., Hauptfeld, V. & Hauptfeld, M. Evidence for a fifth (G) region in theH-2 complex of the mouse. Immunogenetics 2, 141–150 (1975). https://doi.org/10.1007/BF01572282
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DOI: https://doi.org/10.1007/BF01572282