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DNA-DNA homology studies among strains ofHaloferax and other halobacteria

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Abstract

Deoxyribonucleic acid (DNA)-DNA hybridization was used to determine the relationships of the type strains of the three species of the genusHaloferax to 21 halobacterial strains representing isolates from hypersaline habitats and collection strains. Two genotypic groups with high DNA homology were obtained, belonging to the speciesHaloferax gibbonsii andHaloferax mediterranei. The highest DNA relatedness (≥74%) was obtained in theHalobacterium salinarium group.Halobacterium denitrificans showed very low DNA homology with the other halobacteria investigated. The G+C content of the eight strains ofHaloferax tested ranged between 59.1 and 65.5 mol%.

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Literature Cited

  1. De Ley J, Tijtgat R (1970) Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36:461–474

    PubMed  Google Scholar 

  2. Ferragut C, Leclerc H (1976) Etude comparative des méthodes de détermination du Tm de l'ADN bactérien. Ann Microbiol 127:223–235

    Google Scholar 

  3. Grimont PAD (1984) DNA/DNA hybridization in bacterial taxonomy.In: Sanna A, Morace G (eds), New horizonts in microbiology. Amsterdam: Elsevier, pp 11–19

    Google Scholar 

  4. Gutiérrez MC, García MT, Ventosa A, Nieto JJ, Ruiz-Berraquero F (1986) Occurrence of megaplasmids in halobacteria. J Appl Bacteriol 61:67–71

    Google Scholar 

  5. Johnson JL (1981) Genetic characterization.In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB (eds) Manual of methods for general bacteriology. Washington: American Society of Microbiology, pp 450–472

    Google Scholar 

  6. Juez G, Rodriguez-Valera F, Ventosa A, Kushner DJ (1986)Haloarcula hispanica spec. nov. andHaloferax gibbonsii spec. nov., two species of extremely halophilic archaebacteria. Syst Appl Microbiol 8:75–79

    Google Scholar 

  7. Kushner DJ, Kamekura M (1988) Physiology of halophilic eubacteria,In: Rodriguez Valera F (ed), Halophilic bacteria, vol 1. Boca Raton FL: CRC Press, pp 109–138

    Google Scholar 

  8. Larsen H (1984) Family Halobacteriaceae,In: Krieg NR, Holt JG (eds), Bergey's manual of systematic bacteriology, vol. 1. Baltimore: Williams and Wilkins, pp 261–267

    Google Scholar 

  9. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218

    Google Scholar 

  10. Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    PubMed  Google Scholar 

  11. Moore RL, McCarthy BJ (1969) Characterization of the deoxyribonucleic acid of various strains of halophilic bacteria. J Bacteriol 99:248–254

    PubMed  Google Scholar 

  12. Oren A (1983)Halobacterium sodomense spec. nov., a Dead SeaHalobacterium with an extremely high magnesium requirement. Int J Syst Bacteriol 33:381–386

    Google Scholar 

  13. Owen RJ, Hill LR (1979) The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids.In: Skinner FA, Lovelock DW (eds), Identification methods for microbiologists, 2nd ed. London: Academic Press, pp 217–296

    Google Scholar 

  14. Owen RJ, Pitcher D (1985) Current methods for estimating DNA base composition and levels of DNA-DNA hybridization,In: Goodfellow M, Minnikin E (eds), Chemical methods in bacterial systematic. London: Academic Press, pp 67–93

    Google Scholar 

  15. Pfeifer F (1988) Genetics of halobacteria, In: Rodriguez-Valera F (ed), Halophilic bacteria, vol 2. Boca Raton, FL: CRC Pres, pp 105–133

    Google Scholar 

  16. Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A (1980) Behaviour of mixed populations of halophilic bacteria in continuous culture. Can J Microbiol 26:1259–1263

    PubMed  Google Scholar 

  17. Ross HNM, Grant WD (1985) Nucleic acid studies on halophilic archaebacteria. J Gen Microbiol 131:165–173

    PubMed  Google Scholar 

  18. Sapienza C, Doolittle W (1982) Repeated sequences in the genome of halobacteria. Zentralbl Bakteriol Mikrobiol Hyg C 3:120–127

    Google Scholar 

  19. Soliman GSH, Trüper HG (1982)Halobacterium pharaonis sp. nov., a new extremely haloalkaliphilic archaebacterium with low magnesium requirement. Zentralbl Bakteriol Mikrobiol Hyg C 3:318–329

    Google Scholar 

  20. Tindall BJ, Ross HNM, Grant WD (1984)Natronobacterium gen. nov. andNatronococcus gen. nov., two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5:41–57

    Google Scholar 

  21. Tomlinson GA, Jahnke LL, Hochstein LI (1986)Halobacterium denitrificans sp. nov., an extremely halophilic denitrifying bacterium. Int J Syst Bacteriol 36:66–70

    PubMed  Google Scholar 

  22. Torreblanca M, Rodriguez-Valera F, Juez G, Ventosa A, Kamekura M, Kates M (1986) Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description ofHaloarcula gen. nov. andHaloferax gen. nov. Syst Appl Microbiol 8:89–99

    Google Scholar 

  23. Ventosa A, Grant WD (1988) International committee on systematic bacteriology, subcommittee on the taxonomy ofHalobacteriaceae. Int J Syst Bacteriol 38:332

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Parasitology, University of Sevilla, Sevilla, Spain

    M. Carmen Gutiérrez,  Antonio Ventosa & Francisco Ruiz-Berraquero

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  1. M. Carmen Gutiérrez
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  2. Antonio Ventosa
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  3. Francisco Ruiz-Berraquero
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Gutiérrez, M.C., Ventosa, A. & Ruiz-Berraquero, F. DNA-DNA homology studies among strains ofHaloferax and other halobacteria. Current Microbiology 18, 253–256 (1989). https://doi.org/10.1007/BF01570301

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