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Entrainment and phase shifting of the circadian rhythm of cell division by light in cultures of the achlorophyllous ZC mutant ofEuglena gracilis

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Abstract

The photosynthesis-deficient ZC mutant ofEuglena gracilis Klebs (strain Z) was cultured at 16°C on an aerated, magnetically stirred, mineral medium containing 0.1% ethanol (pH 7.0). Cell division could be entrained by a 12: 12 light: dark cycle (LD: 12, 12) or even by a one-pulse skeleton photoperiod (LD: 1,23) The rhythm free-ran in DD for at least 8 days with a circadian period (τ=25.5 h) in populations that had been previously entrained by LD. The freerunning rhythm could be phase-shifted by a single 1-h light pulse (3000 lx). The ‘strong’ (Type 0) phase-response curve derived from the resetting effects of such signals given at different circadian times was similar to that for the photosynthetic wild-type strain. These results demonstrate that the presence of a functional chloroplast compartment is not necessary for the circadian clock to function inEuglena and suggest that phase resetting of the circadian clock by light occurs via a similar pathway in both photosynthetic and non-photosynthetic cell types.

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Authors and Affiliations

  1. Department of Anatomical Sciences, State University of New York, Stony Brook, New York, USA

    Isabelle A. Carré, Ady S. Oster, Danielle L. Laval-Martin & Leland N. Edmunds Jr.

  2. Laboratory of Biological Membranes, University of Paris, Paris, France

    Isabelle A. Carré & Danielle L. Laval-Martin

Authors
  1. Isabelle A. Carré
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  2. Ady S. Oster
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  3. Danielle L. Laval-Martin
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  4. Leland N. Edmunds Jr.
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Carré, I.A., Oster, A.S., Laval-Martin, D.L. et al. Entrainment and phase shifting of the circadian rhythm of cell division by light in cultures of the achlorophyllous ZC mutant ofEuglena gracilis . Current Microbiology 19, 223–229 (1989). https://doi.org/10.1007/BF01570166

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