Summary
Milbemycin production byStreptomyces hygroscopicus RB4569D was examined in media containing different carbohydrates. Total milbemycin titer could be increased by substitution of fructose for glucose and by selection of the appropriate starch type. Total titer could be further enhanced by increasing the concentration of fructose and/or starch in the medium. Rates of carbohydrate utilization were shown to be independent of their initial concentration and increased titers in high carbohydrate media were shown to be due to a prolonged production phase rather than an increased accretion rate. The pattern of individual milbemycin components was governed by the carbon:nitrogen ratio of the medium rather than carbohydrate concentration and there was a critical C:N ratio below which no milbemycin was produced.
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Warr, S.R.C., Box, S.J., Burbidge, C. et al. Milbemycin production byStreptomyces sp.: the effect of carbohydrates. Journal of Industrial Microbiology 13, 43–48 (1994). https://doi.org/10.1007/BF01569661
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DOI: https://doi.org/10.1007/BF01569661