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Assignment of a structural gene forβ-glucuronidase to human chromosome C7

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Somatic Cell Genetics

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Abstract

An electrophoretic technique was developed which allows the separation of human β-glucuronidase (GUS EC 3.2.1.31) from the enzyme present in cultured murine, Chinese and Syrian hamster cells in one buffer system on Cellogel. Using this technique a number of independent human-mouse somatic cell hybrids have been analyzed for the segregation of GUS, other enzyme markers, and all human chromosomes. The results indicate that a structural gene for human β-glucuronidase is located on chromosome C7.

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Literature cited

  1. De Duve, C., Pressman, B. C., Gianetto, R., Wattiaux, R., and Appelmans, F. (1955).Biochem. J. 60:604–617.

    PubMed  Google Scholar 

  2. Fishman, W. H., Goldman, S. S., and Delellis, R. (1967).Nature 213:457–460.

    PubMed  Google Scholar 

  3. Paigen, K. (1961).Exp. Cell Res. 25:286–301.

    PubMed  Google Scholar 

  4. Paigen, K., Swank, R. T., Tomino, S., and Ganschow, R. E. (1975).J. Cell. Physiol. 85:379–392.

    PubMed  Google Scholar 

  5. Lalley, P. A., and Shows, T. B. (1974).Science 185:442–444.

    PubMed  Google Scholar 

  6. Glaser, J. H., Roozen, K. J., Brot, F. E., and Sly, W. S. (1975).Arch. Biochem. Biophys. 166:536–542.

    PubMed  Google Scholar 

  7. Nichol, D. M., Lagunoff, D., and Pritzl, P. (1974).Biochem. Biophys. Res. Commun. 59:941–946.

    PubMed  Google Scholar 

  8. Higashino, K., Okochi, T., Aoki, T., Ito, F., and Yamamura, J. (1971).Med. J. Osaka Univ. 21:251–255.

    PubMed  Google Scholar 

  9. Sly, W. S., Quinton, B. A., McAllister, W. H., and Rimoin, D. L. (1973).J. Pediatr. 82:249–257.

    PubMed  Google Scholar 

  10. Hall, C. W., Cantz, M., and Neufeld, E. D. (1973).Arch. Biochem. Biophys. 155:32–38.

    PubMed  Google Scholar 

  11. McKusick, V. A. (1972).Hereditable Disorders of Connnective Tissues. Mosby, St. Louis.

    Google Scholar 

  12. Brot, F. E., Glaser, J. H., Roozen, K. J., and Sly, W. S. (1974).Biochem. Biophys. Res. Commun. 57:1–8.

    PubMed  Google Scholar 

  13. Klebe, R. J., Chen, T. R., and Ruddle, F. (1970).J. Cell Biol. 45:74–82.

    PubMed  Google Scholar 

  14. Littlefield, J. W. (1966).Exp. Cell Res. 41:190–196.

    PubMed  Google Scholar 

  15. Grzeschik, K.-H. (1973).Humangenetik 20:211–218.

    PubMed  Google Scholar 

  16. Meera Khan, P. (1971).Arch. Biochem. Biophys. 245:470–483.

    Google Scholar 

  17. Grzeschik, K.-H. (1973). Habilitationsschrift (Universität Münster).

  18. Van Someren, H., Beyersbergen van Hengenouwen, H., Los, W. R. T., Wurzer-Figurelli, E. M., Doppert, B. A., Vervloet, M., and Meera Khan, P. (1974).Humangenetik 25:190–210.

    Google Scholar 

  19. Caspersson, T., Zech, L., and Johansson, C. (1970).Exp. Cell Res. 60:315–319.

    PubMed  Google Scholar 

  20. Kim, My. A., and Grzeschik, K.-H. (1974).Exp. Cell Res. 88:406–410.

    PubMed  Google Scholar 

  21. Creagan, R., Tischfield, J., McMorris, F. A., Chen, S., Hirschi, M., Chen, T. R., Ricciuti, F., and Ruddle, F. H. (1973).Cytogenet. Cell Genet. 12:187–198.

    PubMed  Google Scholar 

  22. Croce, C. M., Aden, D., and Koprowski, H. (1975).Science 190:1200–1202.

    PubMed  Google Scholar 

  23. Croce, C. M., and Koprowski, H. (1974).J. Exp. Med. 140:1221–1229.

    PubMed  Google Scholar 

  24. Keller, R. K., and Touster, O. (1975).J. Biol. Chem. 250:4765–4769.

    PubMed  Google Scholar 

  25. Gilbert, F., Kucherlapati, R., Murnane, M. F., Darlington, G. F., Creagan, R., and Ruddle, F. H. (1974).Birth Defects: Original Article Series, Vol. 10, No. 3: pp. 96–99, The National Foundation, New York.

    Google Scholar 

  26. Van Heyningen, V., Bobrow, M., Bodmer, W. F., Povey, S., Gardiner, S. E., and Hopkinson, D. A. (1975).Birth Defects: Original Article Series, Vol. 6, No. 3: pp. 183–187, The National Foundation, New York.

    Google Scholar 

  27. Grzeschik, K.-H. (1975). Baltimore Conference, Third International Workshop on Human Gene Mapping.

  28. Croce, C. M., Girardi, A. J., and Koprowski, H. (1973).Proc. Natl. Acad. Sci. U.S.A. 70:3617–3620.

    PubMed  Google Scholar 

  29. Womack, T. E., Hawles, N. L., Soures, E. R., and Roderick, T. H. (1975).Biochem. Genet. 13:519–526.

    PubMed  Google Scholar 

  30. Lalley, P. A., Brown, J. A., Eddy, R. L., Haley, L. L., and Shows, T. B. (1975). Baltimore Conference, Third International Workshop on Human Gene Mapping.

  31. Murnane, M. J., Kucherlapati, R. S., Chen. S., and Ruddle, F. H.Am. J. Hum. Genet. 27:68A.

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  1. Institut für Humangenetik der Universität Münster, Münster/Westf, Germany

    Karl-Heinz Grzeschik

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  1. Karl-Heinz Grzeschik
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Grzeschik, KH. Assignment of a structural gene forβ-glucuronidase to human chromosome C7. Somat Cell Mol Genet 2, 401–410 (1976). https://doi.org/10.1007/BF01542721

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  • DOI: https://doi.org/10.1007/BF01542721

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