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Half-life of polyreactive antibodies

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Abstract

Monoclonal polyreactive antibodies bind to a variety of self and foreign antigens. In contrast, monoclonal monoreactive antibodies bind to a single or restricted number of known antigens. The rate at which polyreactive antibodies are removed from the circulation compared to monoreactive antibodies has not been determined. In the present experiments, human monoclonal polyreactive and monoreactive antibodies of different isotypes were injected intravenously into mice and the clearance from the circulation was determined. The halflife of polyreactive IgM, IgA, and IgG antibodies was 8.0, 8.2, and 9.8 hr, respectively, compared to 35.4, 26.6, and 280 hr for monoreactive IgM, IgA, and IgG antibodies, respectively. Examination of tissue sections from animals given intravenous antibody showed substantial deposition of polyreactive, but not monoreactive, antibodies in several organs, the liver being the principal site of deposition. It is concluded that polyreactive antibodies are cleared from the circulation substantially faster than monoreactive antibodies.

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Author notes

  1. Nagaradona Harindranath

    Present address: Center for Biologics Evaluation and Research, Food and Drug Administration, Building 29A, Room 2B-09, 20892, Bethesda, Maryland

Authors and Affiliations

  1. Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Building 30, Room 121, 20892, Bethesda, Maryland

    George Sigounas, Nagaradona Harindranath, Giulia Donadel & Abner Louis Notkins

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  1. George Sigounas
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  2. Nagaradona Harindranath
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  3. Giulia Donadel
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  4. Abner Louis Notkins
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Sigounas, G., Harindranath, N., Donadel, G. et al. Half-life of polyreactive antibodies. J Clin Immunol 14, 134–140 (1994). https://doi.org/10.1007/BF01541346

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