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Lipopolysaccharide priming of superoxide release by human neutrophils: Role of membrane CD 14 and serum LPS binding protein

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Abstract

Previous studies have suggested that lipopolysaccharide (LPS) interactions with neutrophils and monocytes are mediated via the CD14 receptor, in the presence of serum factors such as LPS-binding protein (LBP) and septin. The present study was designed to test if CD14-mediated LPS priming of human neutrophils is dependent upon the presence of serum proteins and to evaluate the contribution of serum factors in LPS-neutrophil interactions. The results demonstrate that CD14 mediates the priming of neutrophil superoxide release by LPS both in the presence and in the absence of serum. However, priming by LPS is greatly enhanced in the presence of human serum, and the factor responsible for this phenomenon is LBP and not heat-sensitive proteins, such as septin.

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Authors and Affiliations

  1. Department of Periodontics, Hebrew University-Hadassah Faculty of Dental Medicine, P.O. Box 12272, 91120, Jerusalem, Israel

    Lior Shapira

  2. Department of Periodontology, Eastman Dental Center, Rochester, New York

    Lior Shapira, Catherine Champagne, Barbara Gordon, Salomon Amar & Thomas E. Van Dyke

Authors
  1. Lior Shapira
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  2. Catherine Champagne
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  3. Barbara Gordon
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  4. Salomon Amar
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  5. Thomas E. Van Dyke
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Shapira, L., Champagne, C., Gordon, B. et al. Lipopolysaccharide priming of superoxide release by human neutrophils: Role of membrane CD 14 and serum LPS binding protein. Inflammation 19, 289–295 (1995). https://doi.org/10.1007/BF01534388

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