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Feulgenhydrolysekinetik in Eu- und Heterochromatin

Feulgen hydrolysis kinetics in euchromatin and heterochroniatin

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Summary

Feulgen hydrolysis kinetics were investigated in euchromatin and heterochromatin separately using forebrain ganglionar cells of the field vole, Microtus agrestis L. In females of this species two large heterochromatic blocs are formed by the huge X-chromosomes containing 20% of the cell's DNA content and occupying 5.5% of the nuclear surface. The cells were stained according to the Feulgen procedure following different hydrolysis times. Absorbance data were extracted from the microscopic images by the scanning microphotometer UMSP I, punched into paper tape and fed to an IBM 1130 computer. Data were processed for the separation of grid points belonging to heterochromatin and euchromatin, respectively. “Heterochromatin points” were defined to be the points with the highest absorbance and forming 5.5% of the nuclear surface area, the rest being euchromatin.

For each chromatin fraction the total dye content was calculated. The proportion of dye amount in the heterochromatin part was found to be 20% of the total cell nucleus and is independent of hydrolysis time. Hydrolysis kinetics in both euchromatin and heterochromatin are identical. It is suggested that “atypeness” of hydrolysis curves are not due to different hydrolysis properties of diffuse and condensed chromatin.

Zusammenfassung

Von Feulgengefärbten Ganglienzellkernen der Erdmaus,Microtus agrestis L., wurden die cytophotometrisch gemessenen DNA-Werte auf Lochstreifen gestanzt. Die beiden Kernbezirke, der dem Eu- und der dem Heterochromatin angehörende, wurden durch bildanalytische Auswertung (picture processing) des mikroskopischen Kernbildes mit einer Datenverarbeitungsanlage getrennt. Es konnten keine Unterschiede im Verlauf der Hydrolysekinetik gefunden werden.

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Authors and Affiliations

  1. Pathologisches Institut (Ludwig Aschoff-Haus) der Universität Freiburg i. Br., Deutschland

    Renate Kiefer, Günter Kiefer & Walter Sandritter

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  1. Renate Kiefer
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  2. Günter Kiefer
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  3. Walter Sandritter
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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg (SFB 46).

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Kiefer, R., Kiefer, G. & Sandritter, W. Feulgenhydrolysekinetik in Eu- und Heterochromatin. Histochemie 30, 150–155 (1972). https://doi.org/10.1007/BF01444061

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  • DOI: https://doi.org/10.1007/BF01444061

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