Summary
We describe our protocol for isolating myogenic cells from the semimembranous muscles of lamb. Cultured cells display properties similar to myogenic satellite cells isolated from other species; similar characteristics include the ability to proliferate, differentiate, and fuse to form myotubes in primary cell culture. Using the described procedures, we provide the first documentation of satellite like cells existing in postnatal skeletal muscle of a ruminant animal species.
Similar content being viewed by others
References
Allen, R. E.; Dodson, M. V.; Luiten, L. S. Regulation of skeletal muscle satellite cell proliferation by bovine pituitary fibroblast growth factor. Exp. Cell Res. 152:154–160; 1984.
Allen, R. E.; Luiten, L. S.; Dodson, M. V. Effect of insulin and linoleic acid on satellite cell differentiation. J. Anim. Sci. 60:1571–1579; 1985.
Bischoff, R. Enzymatic liberation of myogenic cells from adult rat muscle. Anat. Rec. 180:645–661; 1974.
Bischoff, R. Proliferation of muscle satellite cells on intact myofibers in culture. Dev. Biol. 115:129–139; 1986.
De La Haba, G.; Cooper, G. W.; Elting, V. Hormonal requirements for myogenesis of striated muscle in vitro: Insulin and somatotropin. Proc. Natl. Acad. Sci. USA 56:1719–1723; 1966.
Dodson, M. V.; Allen, R. E.; Hossner, K. L. Ovine somatomedin, multiplication-stimulating activity and insulin promote skeletal muscle satellite cell proliferation in vitro. Endocrinology 117:2357–2363; 1985.
Florini, J. R.; Nicholson, M. L.; Dulak, N. C. Effects of peptide anabolic hormones on growth of myoblasts in culture. Endocrinology 104:32–41; 1977.
Florini, J. R.; Ewton, D. Z. Insulin acts as a somatomedin analog in stimulating myoblast growth in serum-free medium. In Vitro 17:763–768; 1981.
Florini, J. R. Hormonal control of muscle cell growth. J. Anim. Sci. 61:21–38; 1985.
Hausman, R. E.; Vellman, S. G. Prostaglandin E1 receptors on chick embryo myoblasts. Biochem. Biophys. Res. Comm. 103:213–218; 1981.
Kumegawa, M.; Ikeda, S.; Takuma, T. In vitro effects of thyroxine and insulin on myoblasts from chick embryo skeletal muscle. Dev. Biol. 79:493–499; 1980.
Mandel, J.-L.; Pearson, M. L. Insulin stimulates myogenesis in a rat myoblast line. Nature 251:618–620; 1974.
Mauro, A. Satellite cell of skeletal muscle fibers. Biochem. Biophys. Cytol. 9:493–496; 1961.
Sandra, A.; Przybylski, R. J. Ontogeny of insulin binding during chick skeletal myogenesis in vitro. Dev. Biol. 68:546–556; 1979.
Schmid, C.; Steiner, T.: Froesch, E. Preferential enhancement of myoblast differentiation by insulin-like growth factors (IGF-I and IGF-II) in primary cultures of chicken embryonic cells. FEBS. Lett. 161:117–121; 1983.
Stockdale, F. E.; Holtzer, H., DNA synthesis and myogenesis. Exp. Cell Res. 24:508–520; 1961.
Tauro, K. A.; Florini, J. R. Hormonal stimulation of myoblast differentiation in the absence of DNA synthesis. Am. J. Physiol. 243:C278-C284; 1982.
Author information
Authors and Affiliations
Additional information
Technical assistance provided.
Rights and permissions
About this article
Cite this article
Dodson, M.V., McFarland, D.C., Martin, E.L. et al. Isolation of satellite cells from ovine skeletal muscles. Journal of Tissue Culture Methods 10, 233–237 (1986). https://doi.org/10.1007/BF01404483
Issue Date:
DOI: https://doi.org/10.1007/BF01404483