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Microtubules in pollen tube subprotoplasts: organization during protoplast formation and protoplast outgrowth

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Summary

Microtubules inNicotiana tabacum pollen tube subprotoplasts reassembled in wave-like to concentric cortical arrays. Crosslinks between microtubules were either 15 or 80 nm in length. Cortical actin filaments showed different distributions; no colocalization like that in pollen tubes was observed. Degradation of actin filaments by cytochalasin D had no influence on microtubule organization. Degradation of microtubules and/or actin filaments did not affect outgrowth of the subprotoplasts. Organization of the microtubules occurred independent of the presence of the generative cell and/or the vegetative nucleus. No relation of actin filament and microtubule organization with organelle distribution could be detected.

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Abbreviations

AFs:

actin filaments

DAPI:

4′,6-diamidino-2-phenylindole

EGTA:

ethylene glycol bis (2-amino ethylether) N,N,N′,N′-tetraacetic acid

FITC:

fluorescein isothiocyanate

MTs:

microtubules

SPPs:

subprotoplasts

TRITC:

tetramethyl rhodamine B isothiocyanate

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Authors and Affiliations

  1. Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525, ED Nijmegen, The Netherlands

    T. L. M. Rutten & J. Derksen

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  1. T. L. M. Rutten
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  2. J. Derksen
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Rutten, T.L.M., Derksen, J. Microtubules in pollen tube subprotoplasts: organization during protoplast formation and protoplast outgrowth. Protoplasma 167, 231–237 (1992). https://doi.org/10.1007/BF01403386

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  • DOI: https://doi.org/10.1007/BF01403386

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