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Die Wirkung von Röntgenstrahlen auf das Transmembranpotential und die elektrophoretische Beweglichkeit polymorphkerniger Granulozyten und FL-Zellen

The effect of roentgen rays on the transmembrane potential and on the electrophoretic mobility of polymorphonuclear granulocytes and FL-cells

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Summary

Two hours after X-irradiation at 37° C in vitro at doses ranging from 50 rad to 20 krad the transmembrane potential and the surface charge of polymorphonuclear granulocytes andFL-cells showed a dose-dependent decrease. However, between two and four days after irradiation at 10 kradFL-cells revealed an increase in both transmembrane potential and surface charge. Small doses of ionizing radiation (e.g. 10 rad) resulted in aperiodically damped oszillations of membrane polarisation in leucocytes.

Zusammenfassung

Zwei Stunden nach Röntgenbestrahlung bei 37° C in vitro mit Dosen zwischen 50 rad and 20 krad sind das Transmembranpotential and die Oberflächenladung von polymorphkernigen Granulozyten andFL-Zellen dosisabhängig erniedrigt. Zwei bis vier Tage nach einer Bestrahlung mit 10 krad habenFL-Zellen jedoch ein erhöhtes Transmembranpotential und eine erhöhte Oberflächenladung. Kleine Dosen ionisierender Strahlung (z. B. 10 rad) lösen bei Leukozyten aperiodisch gedämpfte Schwingungen der Membranpolarisation aus.

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Literatur

  1. Allen, N., Nicholls, J. G.: A study of effect of x-rays on the electrical properties of mammalian nerve and muscle. Proc. roy. Soc. B157, 536–561 (1963)

    Google Scholar 

  2. Aull, F.: Measurement of the electrical potential difference across the membrane of Ehrlich Mouse Ascites tumor cell. J. cell. Physiol.69, 21–32 (1967)

    Google Scholar 

  3. Bachofer, C. S., Gautereaux, M. E.: Bioelectric activity of mammalian nerves during x-irradiation. Radiat. Res.12, 575–586 (1960)

    Google Scholar 

  4. Beckmann, A., Jenssen, H. L., Kalkoff, W., Redmann, K.: Das bioelektrische Potential an der zytoplasmatischen Membran der Granulozyten. Experientia (Basel)26, 186 (1970)

    Google Scholar 

  5. Bergeder, H. D.: Kaliumverluste von Kaltblütermuskeln nach Röntgenbestrahlung. Naturwissenschaften45, 61 (1958)

    Google Scholar 

  6. Bresciani, F., Aurichio, F., Fiore, C.: Effect of x-rays on movements of sodium in human erythrocytes. Radiat. Res.21, 394 (1964)

    Google Scholar 

  7. Dawson, K. B., Seymour, R., Mutton, D. E.: Radiation-induced efflux of intracellular potassium. Radiat. Res.37, 83–89 (1969)

    Google Scholar 

  8. Dolphin, G. W., Purrott, R. J.: Use of radiation-induced chromosome aberrations in human lymphocytes for dosimetrie. Reprint from Advances in Physical and Biological Detectors. International Atomic Energy Agency, IAEA-SM, p. 143, Vienna 1971

    Google Scholar 

  9. Esch, H., Miltenberger, H., Hug, O.: Die Beeinflussung elektrischer Potentiale von Algenzellen durch Röntgenstrahlen. Biophysik1, 380–388 (1964)

    Google Scholar 

  10. Franke, H. D., Lierse, W.: Early changes in ultrastructure of glial cells and myelinization in rat brain after x-irradiation. Fortschr. Röntgenstr.107, 415–420 (1967)

    Google Scholar 

  11. Fuhrmann, G. F., Ruhenstroth-Bauer, G.: Cell electrophoresis employing a rectangular measuring cuvette. In: Cell electrophoresis. (Ambrose, E. J., Ed.). Boston: Little & Brown 1965

    Google Scholar 

  12. Gaffey, C. T.: The response of maximal and submaximal action potentials from frog sciatic nerve to 200 kV x-rays. Radial. Res.45, 311–325 (1971)

    Google Scholar 

  13. Gaffey, C. T.: Stimulation of action potentials with radiation in single cells of Nitella gracilis. Int. J. Radial. Biol.21, 11–29 (1972)

    Google Scholar 

  14. Günther, J., Hug, O.: Akute Wirkungen von Röntgenstrahlen auf Membranpotential und -widerstand von Schneckenneuronen. Rad. Environm. Biophys.11, 171–187 (1974)

    Google Scholar 

  15. Hasl, G., Pauly, H.: Elektrolyttransport in Ehrlich-Aszitestumorzellen nach Röntgenbestrahlung. I. Bestrahlung dichtgepackter Zellen. Biophysik5, 223–236 (1968)

    Google Scholar 

  16. Hasl, G., Pauly, H.: Elektrolyttransport in Ehrlich-Aszitestumorzellen nach Röntgenbestrahlung. II. Bestrahlung von Zellsuspensionen. Biophysik5, 237–247 (1968)

    Google Scholar 

  17. Haydon, D. A.: The surface charge of cells and some other small particles as indicated by electrophoresis. I. The zeta potential-surface charge relationships. Biochim. biophys. Acta (Amst.)50, 450–457 (1961)

    Google Scholar 

  18. Helmholtz, H.: Studien über elektrische Grenzschichten. Ann. Phys.7, 337 (1879)

    Google Scholar 

  19. Hildebrand, E.: Untersuchungen zur Wirkung ultravioletten Lichtes auf die elektrischen Eigenschaften der Zellmembran einiger Protozoen. Dissertation, TH Aachen 1969

  20. Hollingshead, S., Thomason, D.: Effect of radiation on the electrophoretic mobility of Ehrlich ascites tumour cells. Nature (Lond.)195, 1217 (1962)

    Google Scholar 

  21. Hug, O., Schliep, H. J.: Immediate reactions of nerves and muscles to ionizing radiation. Symp. on the initial effects of ionizing radiations on cells, Moscow, pp. 287–300 (1960). London-New York: Academic Press 1961

    Google Scholar 

  22. Kim, S. H., Kim, J. H., Djordjevic, B.: Effects of x-irradiation on RNA and protein synthesis in Hela cells. Radiat. Res.42, 577 (1970)

    Google Scholar 

  23. Lebedinsky, A. V., Grigoryev, U. G., Demirchoglyan, G. G.: On the biological effect of small doses of ionizing radiation. Proc. 2nd U.N. Int. Conf. on Peaceful Uses of Atomic Energy22, 17–28 (1958)

    Google Scholar 

  24. Lehmann, F., Wels, P.: Effect of Roentgen rays on the permeability of red blood cells for erythrocytes. Pflügers Arch. ges. Physiol.213, 628 (1926)

    Google Scholar 

  25. Masurovsky, E. B., Bunge, M. B., Bunge, R. P.: Cytological studies of organotypic cultures of rat dorsal root ganglion following x-irradiation in vitro. II. Changes in Schwann cells, myelin sheaths, and nerve fibres. J. Cell Biol.32, 495–518 (1967)

    Google Scholar 

  26. Morczek, A.: Ionisierende Strahlen und der Haushalt organischer Kationen. Leipzig: Thieme 1957

    Google Scholar 

  27. Morczek, A., Abraham, K.: Zum Mechanismus der Strahlenwirkung auf den K+-Na+-Austausch in Erythrozyten. Biophysik4, 113–117 (1967)

    Google Scholar 

  28. Myers, D. K.: Bide, R. W.: Biochemical effects of x-irradiation on erythrocytes. Radiat. Res.27, 250 (1966)

    Google Scholar 

  29. Myers, D. K.: Some aspects of radiation effects on cell membranes. Advanc. biol. med. Phys.13, 219 (1970)

    Google Scholar 

  30. Portela, A., Hines, M., Perez, J. C., Brandes, D., Bourne, G. H., Stewart, P., Groth, D.: Effects of x-irradiation on muscle membrane. Exp. Cell Res.21, 468–481 (1960)

    Google Scholar 

  31. Prignitz, R., Fröhlich, D., Hoffmeister, G.: Untersuchungen über den Einfluß von Röntgenstrahlen auf Elektrolytverschiebungen und Stoffwechsel des Herzmuskels. VII. Strahlenbedingte Hemmung einer Na+- und K+-aktivierbaren mikrosomalen Transport-ATPase. Strahlentherapie151, 356–365 (1976)

    Google Scholar 

  32. Redmann, K.: Die Verteilung des Membranpotentials in der Zellkultur. Biophysik4, 92–96 (1967)

    Google Scholar 

  33. Redmann, K., Stolte, Ch., Lüders, D.: Membranpotentialmessungen an KB-Zellkulturen. Naturwissenschaften54, 255 (1967)

    Google Scholar 

  34. Redmann, K.: Messungen elektrischer Membranpotentiale kultivierter Einzelzellen mittels Mikroelektroden. Acta biol. med. germ.27, 55–68 (1971)

    Google Scholar 

  35. Redmann, K., Jenssen, H. L., Köhler, H.-J.: Experimental and functional changes in transmembrane potential and zeta potential of single cultured cells. Exp. Cell Res.87, 281–289 (1974)

    Google Scholar 

  36. Redmann, K., Burmeister, J., Jenssen, H. L.: The influence of hyperthermia on the transmembrane potential, zeta-potential and metabolism of polymorphonuclear leucocytes. Acta biol. med. germ.33, 187–196 (1974)

    Google Scholar 

  37. Röttinger, E. M., Hug, O.: The effects of low energy x-rays on membrane potential, membrane resistance and action potential of Nitella flexilis. Radiat. Res.50, 491–503 (1972)

    Google Scholar 

  38. Sato, C., Kojima, K.: Irreversible loss of negative surface charge and loss of colony-forming ability in burkitt lymphoma cells after x-irradiation. Exp. Cell Res.69, 435–439 (1971)

    Google Scholar 

  39. Sato, C., Kojima, K., Matsuzawa, T.: Change in the electrophoretic behavior of x-irradiated lymphocytes by phytohaemagglutinin. Int. J. Radiat. Biol.20, 97–99 (1971)

    Google Scholar 

  40. Shapiro, B., Kollmann, G., Ashen, J.: Mechanism of the effect of ionizing radiation on sodium uptake of human erythrocytes. Radiat. Res.27, 139 (1966)

    Google Scholar 

  41. Shapiro, B., Kollmann, G.: The nature of the membrane injury in irradiated human erythrocytes. Radiat. Res.34, 335 (1968)

    Google Scholar 

  42. Siegel, G.: Die biologischen Wirkungen kleiner Dosen ionisierender Strahlung. Jena: Fischer 1966

    Google Scholar 

  43. Smoluchowski, M.: Contribution a la théorie l'endosmose électrique et de quelques pénomènes correlatifs. Bull. Acad. Sci. Cracovie182 (1903)

  44. Stein, G., Seaman, G. V. F., Heard, D. H.: Action of low doses of ionizing radiations on the electrophoretic mobility of Ehrlich ascites tumour cells. Nature (Lond.)193, 238 (1962)

    Google Scholar 

  45. Taki, M.: Studies on blastogenesis of human lymphocytes by phytohemagglutinin, with special reference to changes of membrane potential during blastoid transformation. Mie med. J.19, 3, 245 (1970)

    Google Scholar 

  46. Tolberg, A. B., Macey, R. J.: The release on membrane-bound calcium by radiation and sulfhydryl reagents. J. Cell Physiol.79, 43 (1972)

    Google Scholar 

  47. Trott, K.-R.: Immediate reactions of potential difference and resistance of the isolated frog skin to X-rays. Int. J. Radiat. Biol.15, 473–482 (1969)

    Google Scholar 

  48. Wallach, D. F. H., Weidekamm, E.: Strahleneffekte an Biomembranen. Klin. Wschr.51, 419–430 (1973)

    Google Scholar 

  49. Woodbury, J. W.: Studies on membrane resting potentials of muscle. Effects of K-lack, x-irradiation and DNP. Exp. Cell Res.5, 547–559 (1958)

    Google Scholar 

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Authors and Affiliations

  1. Abteilung Biophysik und Elektrophysiologie der Zelle und Radiologische Klinik der Medizinischen Akademie Magdeburg, Institut für Physiologie, Leipziger Straße 44, DDR-30, Magdeburg, Deutsche Demokratische Republik

    K. Redmann & G. Reichel

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  1. K. Redmann
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  2. G. Reichel
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Die Arbeit wurde mit Unterstützung des Forschungsverbandes Geschwulsterkrankungen des Ministeriums für Gesundheitswesen der DDR durchgeführt

Eingengangen am 3. Juni 1976

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Redmann, K., Reichel, G. Die Wirkung von Röntgenstrahlen auf das Transmembranpotential und die elektrophoretische Beweglichkeit polymorphkerniger Granulozyten und FL-Zellen. Radiat Environ Biophys 14, 21–30 (1977). https://doi.org/10.1007/BF01331026

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